Koninklijke Philips Corporation N.v.

Prarthana Shrestha

Marius Iosif Boamfa

Jelte Peter Vink

Susanne Maaike Valster

Maarten Van Lierop

Jeroen De Kleijn

Gerardus Johannes Wilhelmus Van Den Braak

Mathijs Rem

A method (and system) is provided obtaining an adapted calibration transformation for whole slide imaging, wherein the adapted calibration transformation is for outputting the color of an empty area of the slide in a whole slide image, WSI, as a desired color. The method comprises first receiving a color target WSI in a device-dependent color space, wherein the color target WSI is a WSI of a color target from a scanner represented in the device-dependent color space. The color target contains at least a white patch and three (near) primary color patches. A calibration transformation is obtained for transforming the device-dependent color space to a device-independent color space. A chromatic adaptation transformation is derived using the color of the white patch in the device-dependent color space, the color of the empty (glass) area in the device-independent color space and the desired empty (glass) color in the target color space. An adapted calibration transformation is obtained by applying the chromatic adaptation transformation to the calibration transformation.

Method and system for whole slide imaging

The present invention relates to digital pathology. In order to improve uniformity at microscopic level and to enhance stability in time for color calibration, a calibration slide () is provided for a digital pathology scanning microscope. The calibration slide comprises a substrate () and a pixel layout () comprising a plurality of spaced apart metal nanostructures () arranged on a surface () of the substrate. The substrate is optically transparent. The metal nanostructures are arranged to produce plasmon resonances for generating a color image under a bright-field illumination. The color image comprises a plurality of calibration color values that are provided for calibrating a digital pathology imaging system.

Calibration slide for digital pathology

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Best, Netherlands

Prarthana Shrestha