Bio-rad Laboratories, Inc.

Theranos, Inc.

Pacific Biosciences of California, Inc.

10x Genomics, Inc.

Labrador Diagnostics LLC

N6 Tec, Inc.

Theranos IP Company, LLC

Pranav Patel

Kamila Belhocine

Scott M Tabakman

Aaron Richardson

Josephine Lee

Arkadiusz Bibillo

Keith P Bjornson

Erik Miller

Satwik Kamtekar

Lei Jia

Zahra Kamila Belhocine

Walter H Lee

Indira Wu

Michael Schnall-Levin

Rajiv Bharadwaj

Christopher Hindson

Jeremiah Hanes

Anthony Makarewicz

Andrew D Price

Tobias Daniel Wheeler

Bill Kengli Lin

Yifeng Yin

Mohammad Rahimi Lenji

Benjamin J Hindson

Paul Hardenbol

Katherine Pfeiffer

Fred C Christians

Arek Bibillo

Colleen Cutcliffe

Paul William Wyatt

Elizabeth A Holmes

Christopher V Reggiardo

Cheryl Heiner

Kevin Travers

Clarissa Lui

Jonathan Petersen

Jonas Korlach

Yann Jouvenot

Paul Wyatt

Yassine Kabouzi

Amir Sadri

Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.

Method and system for sampling material from cells

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

Methods for processing nucleic acid samples

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

Methods of cDNA preparation

Modified reverse transcriptases

Hybrid reverse transcriptases are provided that comprise a non-retroviral retrotransposon, or a fragment of the non-retroviral retrotransposon having reverse transcriptase activity, joined to a nucleic acid binding protein. Also provided are methods of using the hybrid reverse transcriptases to prepare a cDNA molecule library.

Hybrid reverse transcriptases

The present disclosure provides methods and systems comprising use of a device. A device may comprise a plurality of locations configured to contain one or more samples, a plurality of thermal elements corresponding and disposed adjacent to the plurality of locations, wherein the plurality of elements are configured to affect a thermal condition within individual locations of the plurality of locations, at least one additional thermal element operably coupled and common to at least a subset of the plurality of elements, wherein the additional thermal element is configured to affect at least one operating condition of the plurality of thermal elements at least partially in response to the thermal condition within the individual locations of the plurality of locations, and at least one optical system configured to detect a signal or change thereof from the individual locations of the plurality of locations, wherein the signal or change thereof is generated at least partially by a change of the thermal condition and is indicative of a property associated with the one or more samples.

Methods and systems for sample analysis

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Recombinant polymerases with increased phototolerance

Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers.

Nucleic acid amplification

The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.

Methods for producing modified reverse transcriptases

Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such nucleic acid polymerases and primers.

This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same

Growing community of inventors

Fremont, CA, United States of America

Pranav Patel