Council of Scientific and Industrial Research

Unichem Laboratories Limited

Indian Veterinary Research Institute

Pradhyumna Kumar Singh

Rakesh Tuli

Samir Vishwanath Sawant

Shiv Kumar Gupta

Praveen Chandra Verma

Chandrashekar Krishnappa

Sharad Saurabh

Rahul Singh

Preeti Rai

Harpal Singh

Santosh Kumar Upadhyay

Dinesh Kumar Yadav

Kuttan Pillai Narayanan Nair

Satish Mishra

Mohammad Shahnawaz

Shadma Ashraf

Kuttan P Narayanan

Ajit P Singh

Manisha Mishra

Ajit Pratap Singh

A novel insecticidal chitinase protein from fernsp., a process for preparation of the insecticidal protein and nucleic acid sequence encoding for said insecticidal protein and its application for insect control purposes.

Insecticidal chitinase protein its encoding nucleotide and application thereof

Nucleic acid sequence encodingleaf agglutinin (AFAL) is disclosed. The invention providesleaf agglutinin (AFAL) recombinant protein, its encoding nucleotides, primers and the process of preparation thereof, said recombinant protein is useful for insect control and haemagglutination activity. AFAL is found more toxic to sap sucking insect pest(cotton aphid) and(whiteflies) as compared to knownleaf agglutinin. AFAL can be used in the development of transgenic plants for resistance against sap sucking and chewing pests.

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A novel chimeric protein of rabies virus designed to express a chimeric G protein at a high level in transgenic plants. A gene was also designed and chemically synthesised to encode the chimeric G protein and expressed at high level in plant tissue. The gene was expressed in transgenic tobacco plants to examine its therapeutic efficacy against infection by rabies virus. The chimeric G protein was enriched in plant membranes. The BalbC mice were immunised with the plant leaf expressed G-protein. Plant derived chimeric G protein elicited higher immune response as compared to the commercial vaccine. The mice displayed protective immunity when they were challenged with live virus. Chimeric G protein expressed at high level in plant leaves was demonstrated to function as a commercially valuable subunit vaccine against rabies virus infection.

Chimeric G protein based rabies vaccine

The invention relates to a chemically synthesized artificial promoter comprising a DNA sequence designed for the target level and pattern of gene expression, by strategically putting together several signature sequences identified by sequence alignment and statistical analysis of a large database constructed for this purpose.

Chemically synthesized artificial promoter for high level expression of transgenes and a method for its synthesis

Chemically synthesized artificial promoter for high level expression of transgenes

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Lucknow, India

Pradhyumna Kumar Singh