A/s Foss Electric

A/s Foss Electric Holding

Poul E Aegidius

Miloslav Zakora

Jorgen F Nielsen

The number of bacteria in a cell population comprising bacteria and somatic cells is determined by concentrating the bacteria and the somatic cells on the surface of a substrate of a filter material, such as a membrane filter, rupturing the somatic cells by treatment with a medium which is not in osmotic balance with the medium inside the cells, such as water which here constitutes a hypotonic medium, and removing or inactivating their ATP, establishing an extraction chamber having an inner wall which is partly defined by the bacteria-bearing surface of the filter material and therein extracting the ATP from the bacteria with a medium containing a surfactant, such as a quaternary ammonium compound, capable of perforating the cell walls of the bacteria, adding luciferin and luciferase and transferring the medium to a measuring chamber, determining the amount of ATP by measuring the light emitted as a result of the luciferin/luciferase reaction and expressing the amount of ATP as the number of bacteria present on the substrate.

Method for detection of bacterial concentration in a sample

The invention relates to a liquid analyzing apparatus, especially for use in determining the fat content of milk samples. The apparatus comprises manually operated dosage syringes or pumps for mixing diluent and liquid sample in a predetermined relationship, a manually operable homogenizer for providing homogenized mixture to photometric measuring means including a cuvette. The homogenizer and the photometric measuring means are arranged within a temperature controlled block or body made from a heat conductive material. The homogenizer may comprise a piston pump having a piston provided with an O-ring and rigidly connected to a piston rod oscillating angularly in operation.

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Helsinge, Denmark

Poul E Aegidius