Third Wave Technologies, Inc.

Poonam Agarwal

Robert W Kwiatkowski

Sarah Olson

Marilyn C Olson-Munoz

Hon Ip

Tamara J Sander

Michelle Curtis

Vecheslav A Elagin

Angela Hudson

Ilse Tyler

Michelle Garsha

Victor I Lyamichev

James Robert Prudent

Jeff G Hall

Mary Ann Brow

David J Marshall

Robert W Kwiatkowski, Jr

Brian Aizenstein

Andrea L Mast

Scott M Law

Susan S Wigdal

Patrick Peterson

Eric B Rasmussen

Nancy Jarvis

Jennifer M Schneiders

Mary C Oldenberg

Carolyn W Miller

Monika De Arruda Indig

Deborah E Burris

Myrta W Atiles

David Arco

Robert W Kwiatowski, Jr

Slava Elagin

Hon S Ip

Kathleen Carroll

Laura Heisler

Molly A Accola

The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.

Detection of HPV

The present invention provides methods, compositions, and kits for detecting the presence or absence of target sequences in a sample, where the sample also contains interfering sequences that are similar or identical to the target sequences. In particular, the present invention provides blocker oligonucleotides that at least partially inhibit the formation of invasive cleavage structures with the interfering sequences but do not substantially inhibit the formation of invasive cleavage structures with the target sequences.

Nucleic acid detection assays employing blocker oligonucleotides

The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5' nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Methods and compositions for detecting target sequences

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Madison, WI, United States of America

Poonam Agarwal