Northeastern University

Other

Trace Bio Analytics, LLC

Akers Biosciences, Inc.

Poguang Wang

Roger W Giese

Raymond F Akers, Jr

Guodong Li

Jianxin Gao

Xin Zhang

Pushkar Mukund Kulkarni

Gang Shao

Michael R MacNeil

Disclosed is a method for detecting the presence of a DNA adduct in DNA. The method involves the pre-labeling of an adducted nucleotide in the DNA with a quaternary ammonium compound.

DNA adductomics by mass tag prelabeling

MALDI-MS operated slightly under matrix storm conditions with a high-energy-transfer, acidic matrix, or these conditions with a high sample to matrix ratio, can with ultrasensitivity detect a nucleobase, modified or canonical, of a nucleic acid species as a jettisoned, protonated molecule.

Jettison-MS for nucleic acid species

The present disclosure relates to a method for detecting a compound, comprising the steps of: contacting a compound with a solid analytical surface (SAS), thereby forming an SAS with an absorbed compound; contacting the SAS with the absorbed compound with a mass tag, wherein the mass tag reacts with the absorbed compound, thereby forming an SAS with a covalently mass-tagged absorbed compound; and detecting the covalently mass-tagged absorbed compound by mass spectrometry. Also disclosed is a device for collecting breath aerosol, comprising a card or an envelope, wherein the card or the envelope comprise a tab, wherein the tab is a SAS.

On-surface mass tagging

Systems and methods employing beds of bagged and caged absorbent and adsorbent materials are disclosed. These inventions are useful in the area of solid phase extraction.

Caged bags of porous materials

The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.

Cationic tags for attomole level detection of analytes by mass spectrometry

Analytical chemical reagents termed non-signal imidazoles and a method for their use that provide a host of advantages for analysis of phosphomonoesters are described. The method and compounds of the invention provide a host of advantages for the analysis of phosphomonoester-containing compounds, namely characteristic, multi-analyte detection with high sensitivity and specificity of known and unknown phosphomonoester-compounds simultaneously.

Non-signal imidazole reagents for mass spectrometry analysis of phosphomonoesters

Ultrasensitive detection of a chemical substance (analyte) by mass spectrometry is achieved by employing a molecular tag that yields an intense parent ion and then an intense daughter ion in a multi-stage mass spectrometer.

Intensified neutral loss tags and use thereof in mass spectrometry

Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##

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Westborough, MA, United States of America

Poguang Wang