Phadia Ab

Gyros Patent Ab

Pirjo Lehtonen

Rudolf Valenta

Peter Valent

Wolfgang R Sperr

Jonas Lidholm

Susanne Spitzauer

Mats Inganäs

Asa Marknell Dewitt

Verena Niederberger

Verena Vienna General Hospital Niederberger

Peter Vienna General Hospital Valent

Susanne c/o Vienna General Hospital Spitzauer

Wolfgang Vienna General Hospital Sperr

Rudolf Vienna General Hospital Valenta

A method of quantitatively determining an analyte in a fluid sample by an immunoassay comprises binding of the analyte to a ligand capable of specifically binding to the analyte, wherein at least part of the analyte is present as an analyte complex is disclosed. The method comprises the steps of: a) subjecting the sample to a first acidic pH to at least substantially dissociate any analyte complex present and provide substantially all analyte in free form, b) raising the first acidic pH to a second acidic pH where re-formation of complexes is prevented but where binding of analyte to the ligand is permitted, and c) determining the binding of analyte to the ligand to quantitatively determine the analyte in the sample.

Method and kit for analyte determination at acidic conditions

The present invention relates to a novel allergen from timothy grass () pollen, Phl p11 as disclosed in SEQ ID NO:2, and use thereof as a reagent and in a diagnositic kit as well as for immunotherapy.

Allergen

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Uppsala, Sweden

Pirjo Lehtonen