Jilin University

Pinyi Ma

Dejiang Gao

Daqian Song

Provided is a ratiometric fluorescent probe for detecting aminopeptidase N, and a preparation method and use thereof. In the present disclosure, a Nile blue derivative is adopted as a fluorophore and alanyl is adopted as an identification unit to design and synthesize the ratiometric fluorescent probe NB-APN for detecting APN. After the probe reacts with APN, the NB blocked by alanyl is released, resulting in an increase of a fluorescence peak at 675 nm and a decrease of a fluorescence peak at 610 nm. A ratio signal of the probe exhibits a sensitive response to APN, with a detection limit as low as 15 pg/mL. The probe can be used for quantitative detection of APN in a diluted urine sample, and can also be used for ratiometric fluorescent imaging of APN in a cell model and in vivo fluorescent imaging of APN in a nude mouse tumor model.

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Changchun, China

Pinyi Ma