Chinese Academy of Fishery Sciences

Ping Liu

Jian Li

Xianyun Ren

Ling Jin

Qingbing Liu

Hongxing Ge

Jianjian Lv

Ruiyong Fan

Baoquan Gao

Weikang Lan

Zhiqiang Chang

Provided is a method of culturingand, which belongs to the technical field of mariculture, including the following steps: preparing a pond and laying a substratum forfrom March 1 to 5; putting theseedlings, and then culturingseedlings on April 1; puttingseedlings on April 15; puttingjuveniles on May 5; harvestingon July 15, and puttingseedlings again; harvestingandfrom November 5 to 10. By adopting the method of biological control of predators of, the use of the pesticides and fishery drugs in the culture and pond-cleaning process is reduced, and green and healthy culture, energy saving and environmental protection are realized.

Method of culturingand

The present invention provides a molecular marker C42257 for rapidly identifying genetic sex ofand applications thereof. The nucleotide sequence of the molecular marker C42257 is shown in SEQ ID NO:1. The nucleotide sequences of the primer pair of molecular marker C42257 used for detection are C42257F: GGGGGACAA ACAGAGACA (SEQ ID NO: 2) and C42257R: ATCGGGGTGGATTTAGAA (SEQ ID NO: 3), respectively. The molecular marker C42257 is not affected by the tissue specificity ofand the environment, and the steps are simple and the results are obvious. The genomic DNA ofand the primer pair are subjected to PCR reaction and electrophoresis detection. If a target band appears at 183 bp, it is a female. The molecular marker can also promote the establishment of the seedling breeding technology of all-female or high-female-ratio. Therefore, it has a broad application prospect.

Molecular marker C42257 for rapidly identifying genetic sex ofand applications thereof

Growing community of inventors

Shandong, China

Ping Liu