Aviva Biosciences Corporation

Ping Lin

Lei Wu

Guoliang Tao

Douglas T Yamanishi

Jia Xu

Jing Cheng

Xiaobo Wang

Junquan Xu

George T Walker

Mingxian Huang

Joe Ouyang

Paul G Hujsak

Sara F Snyder

Andrea Ghetti

Charina Schmitigal

Mengjia Tang

Gioulnar I Harvie

Huimin Tao

David Cerny

Elizabeth Kwok

Wenge Shi

The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.

Methods and compositions for detecting non-hematopoietic cells from a blood sample

Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.

Methods and compositions for detecting rare cells from a biological sample

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Ping Lin