Genereach Biotechnology Corporation

Ping-Hua Teng

Chen Su

Cheng Su

Chien-Chung Jeng

The present disclosure is related to a device for controlling thermal convection velocity of a biochemical reaction. The thermal convection velocity controlling device includes a base body for disposing a tube which is movable, wherein the tube is filled with a buffer of the biochemical reaction; a heating source located at a bottom of the tube or at a side of the tube to heat the buffer; and a flow rate adjusting apparatus for controlling a thermal convection flow direction of the buffer in the tube, whereby the flow rate adjusting apparatus changes a flow velocity and a flow time of the buffer. The present disclosure is also related to a method for controlling thermal convection velocity of a biochemical reaction using the device.

Device for controlling thermal convection velocity of biochemical reaction and method for the same

A reaction tube for performing isothermal polymerase chain reaction therein is provided and includes an upper section, a lower capillary section, a linkage connecting the upper section and the lower capillary section, and a thermal conductor. The lower capillary section has an annealing portion, an annular heating groove, and a close end. The annealing portion is connected with the linkage, and the annular heating groove is connected with the annealing portion. The close end is connected with the annular heating groove. The thermal conductor tightly embraces the annular heating groove of the lower capillary section for conducting heat. Accordingly, a heat generated from the heating source will conduct to the annular heating groove of the lower capillary section of the reaction tube, a temperature gradient along the reaction tube is then provided in order to perform a thermal convection, thereby performing the isothermal polymerase chain reaction in the reaction tube.

Reaction tube for performing isothermal polymerase chain reaction therein

An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat sink and a tube rack. The heat sink has a main body with a through hole for insertion of the test tube. The through hole has a relatively big diameter section and a relatively small diameter section located below the relatively big diameter section. The tube rack is mounted on the heat sink for insertion of the test tube. The apparatus can ensure that the temperature at the liquid level of the reaction mixture is lower than the temperature suitable for conducting the primer annealing step in the polymerase chain reaction process.

Apparatus for insulated isothermal polymerase chain reaction

An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat insulating mount and a heating member. The heat insulating mount has a main body provided with a receiving space for receiving a bottom of the test tube, a lateral channel communicated between the receiving space and an ambient environment, and an upper channel communicated between the receiving space and the ambient environment for insertion of the test tube. The heating member is inserted into the lateral channel for stopping at the bottom of the test tube. The apparatus can minimize the influence caused by the high temperature generated from the heat source on the heat dissipation of the reaction mixture in middle and upper sections of the test tube. The apparatus is suitable for the fluorescent detection of PCR reaction.

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Taichung, Taiwan

Ping-Hua Teng