Inverness Medical Switzerland Gmbh

Sophion Bioscience A/s

Pieter Telleman

Palle Christophersen

Ulrik Darling Larsen

Morten Bech

Soren Peter Olesen

Jorgen Due

Lars Thomsen

Ole Hansen

Anders Wolff

Jon Wulff Petersen

With a method of establishing an enveloped flow in a channel, in the channel an envelopment area () with a channel section () is configured to which a stub with an outlet is connected, where the stub, which can be configured as a polyhedron, is arranged substantially at right-angles to the envelopment area and with the plane of the outlet substantially at right-angles to the channel section. An inlet channel () and an outlet channel () are connected to the envelopment area. By providing a narrowing-down in the cross-section of the channel in immediate extension of the envelopment area (), a hydrodynamic focussing of the sample fluid is achieved when a fluid, such as a sample fluid, is introduced into the stub, and a carrier fluid is introduced into the inlet channel. The advantage of the invention is that since it is necessary only to have two fluid inlets, a relatively simple structure can be established, which can be built up in a monolithic manner, e.g. by injection molding.

Method of establishing at least one enveloped flow in a channel

The present invention relates to a substrate and a method for obtaining an electrophysiological measuring configuration in which a cell forms a high resistive seal (giga-seal) around a measuring electrode making it suitable for determining and monitoring a current flow through the cell membrane. The substrate is typically part of an apparatus for studying electrical events in cell membranes, such as an apparatus for carrying out patch clamp techniques utilised to study ion transfer channels in biological membranes. The substrate has a plurality or an array of measuring sites with integrated measuring and reference electrodes formed by wafer processing technology. The electrodes are adapted to conduct a current between them by delivery of ions by one electrode and receipt of ions by the other electrode and are typically silver/silver halide electrodes. This allows for effective and fast measuring of cells in configurations where the there is a direct electrical connection between the measuring electrode and the cell interior, a whole-cell measuring configuration.

Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels

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Lyngby, Denmark

Pieter Telleman