Danisco US Inc.

Genencor International, Inc.

Genecor International, Inc.

Dupont Nutrition Biosciences Aps

Piet Van Solingen

Nigel S Dunn-Coleman

Craig E Pilgrim

Richard Ray Bott

Wolfgang Aehle

Martijn Scheffers

Paulien Neefe-Kruithof

Brian E Jones

Walter Weyler

Casper Vroemen

Donald E Ward

Wilhelmus A H Van Der Kleij

Michael C Ward

Kathleen A Clarkson

Suzanne E Lantz

Igor Nikolaev

Frits Goedegebuur

Jian Yao

Jayarama K Shetty

Anita Van Kimmenade

Sung Ho Lee

Sander Van Stigt Thans

Jacob Flyvholm Cramer

Donald E Ward, Ii

Neville Marshall Fish

Peter Edvard Degn

Paulien Kruithof

Igor Nikoaev

Suzy Breneman

The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

Glucoamylase variants with altered properties

The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.

Polypeptides having glucoamylase activity and method of producing the same

The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.

Glucoamylase variants

The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.

The present invention is related to glucoamylases having at least 80% sequence identity to aglucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

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The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.

Acid fungal proteases

The invention provides fungal polypeptides fromthat possess anti-microbial activity, polynucleotides encoding the polypeptides, compositions comprising the polypeptides and polynucleotides, and methods of use, thereof.

The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

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Naaldwijk, Netherlands

Piet Van Solingen