Plant Genetic Systems N.v.

Plant Genetic Systems

Bayer Cropscience Ag

Bayer Cropscience Nv

Piet Soetaert

Roel Van Aarssen

Marc Cornelissen

Jan Dockx

Maike Stam

Frank Meulewaeter

Veronique Gossele

Marcus Cornelissen

Klaus Ochmann

Michael Harnau

Bastian Budde

Martin Leube

Methods, kits and compositions of matter are provided which allow amplification of nucleic acid or interest or DNA of interest, comprising the steps of isolating template nucleic acid or DNA from a biological sample using an alkaline extraction solution and directly adding the extract to a reaction mixture, under conditions to amplify the nucleic acids, wherein the isolated template nucleic acid or DNA in the alkaline extraction solution is not diluted or not neutralized prior to the addition to the reaction mixture.

Methods, compositions and devices for amplification of nucleic acids

The invention provides chimeric genes comprising a first promoter recognized by DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA comprising a 5&prime; UTR, an AU-rich heterologous coding sequence, a 3&prime; UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5&prime; region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3&prime; region of gonomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the RNA polymerases corresponding to the used promoters and terminators. Further the invention provides a process for producing a plant expressing a protein or polypeptide encoded by a hoterologous gene which comprises the steps of transforming the nuclear genome of a plant cell with the above-mentioned chimeric genes; and regenerating a transformed plant from the transformed cell.

Gene expression in plants

The present invention provides chimeric genes that comprise a first promoter recognized by a DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA which comprises a 5' UTR, an AU-rich heterologous coding sequence, a 3' UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5' region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3' region of genomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided in the invention are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the RNA polymerases corresponding to the used promoters and terminators. Further the invention provides a process for producing a plant expressing a protein or polypeptide encoded by a heterologous gene which comprises the steps of transforming the nuclear genome of a plant cell with the above-mentioned chimeric genes; and regenerating a transformed plant from the transformed cell.

Modified Bacillus thuringiensis genes with improved expression in plant

A DNA fragment, encoding all or an insecticidally-effective part of a Bt crystal protein, is modified by changing A and T sequences to corresponding G and C sequences encoding the same amino acids, in a region having transcriptional activity less than 25% of the transcriptional activity of a region at the translation initiation site.

Modified Bacillus thuringiensis insecticidal-crystal protein genes and

A DNA fragment, encoding all or an insecticidally-effective part of a Bt crystal protein, is modified by changing A and T sequences to corresponding G and C sequences encoding the same amino acids.

Growing community of inventors

Laarne, Belgium

Piet Soetaert