Centelion

Aventis Pharma S.a.

Rhone-poulenc Rorer S.a.

Gencell, S.a.

Pierre Wils

Daniel Scherman

Joël Crouzet

Béatrice Cameron

Jo&euml;l Crouzet

Françis Blanche

B&eacute;atrice Cameron

Anne-Marie Darquet

Beatrice Cameron

Joel Crouzet

Monique Ollivier

Fran&ccedil;is Blanche

Joel Couzet

A method for double-stranded DNA purification, by which a solution containing DNA in a mixture with other components is passed over a support on which is covalently coupled an oligonucleotide capable of hybridizing with a specific sequence present on the DNA to form a triple helix.

Purification of a triple helix formation with an immobilized obligonucleotide

Purification of a triple helix formation with an immobilized oligonucleotide

Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.

Purification of a triple heli formation with an immobilized oligonucleotide

The invention is directed to a process for purifying double-stranded DNA, comprising using ceramic hydroxyapatite column chromatography. The invention is also directed to a purified recombinant plasmid DNA composition, comprising a chromosomal DNA content that is less than or equal to 0.01% as well as to a composition comprising DNA prepared by the foregoing process.

Purification of plasmid DNA of pharmaceutical quality

Double-stranded DNA molecules characterized in that they are circular and in that they essentially include one or more genes of interest.

Circular DNA expression cassettes for gene transfer

Circular DNA expression cassettes for in vivo gene transfer

Growing community of inventors

Paris, France

Pierre Wils