Transgene S.a.

Pierre Meulien

Andrea Pavirani

Frederic Perraud

Sophie Jallat

Cell lines have been prepared from transgenic mice comprised of a first exogenous DNA sequence encoding human factor IX which is controlled by a promoter region specific for hepatic cells and a second exogenous DNA sequence encoding an oncogene, such as the SV40 virus gene encoding the T-antigen or the mouse c-myc gene, which is controlled by a promoter region specific for hepatic cells. Promoter regions specific for hepatic cells included the promoter region for the factor IX gene and the promoter region for the .alpha.-1 antitrypsin gene. The cultured cells produce biologically active human factor IX which is then isolated from the culture media.

Cell lines from a transgenic mouse which express biologically active IX

The present invention relates to a novel DNA sequence coding for factor IX or a similar protein, corresponding to a prosequence and to mature factor IX or the mature similar protein. According to the invention, position (-3) in the prosequence is occupied by a codon coding for valine, arginine, lysine, threonine or serine, and/or the first codon of the sequence coding for the mature protein codes for an alanine.

DNA sequence coding for human factor IX or a similar protein, expression

Factor VIII analog which has undergone deletion of amino acids 771 to 1666, prepared from eukaryotic cells transformed by an expression vector carrying the cDNA of the factor VIII which has undergone deletion.

Growing community of inventors

Strasbourg, France

Pierre Meulien