Lyncee Tec Sa

Ecole Polytechnique Federale De Lausanne

Universite De Lausanne /Bra

Université De Lausanne

Ecole Polytechnique Féderale De Lausanne (Epfl)

Pierre Marquet

Christian Depeursinge

Etienne Cuche

Pierre Julius Magistretti

Pascal Jourdain

Nicolas Pavillon

Jonas Kühn

Yves Emery

Frédéric Montfort

Pierre Magistretti

Tristan Colomb

Nicolas Aspert

Jonas Kuehn

Florian Charriere

Anca Marian

Sébastien Bourquin

Silvain Herminjard

Mihaela Anca Marian

An apparatus for measuring through optical means temporally resolved, optical properties, and/or phenotypes, linked to cellular homeostasis. Those temporal measurements enable the detection of cell regulation through various channels linked to homeostasis, in order to assess cell viability or early cell death through rapid diagnostic.

Apparatus and method for early diagnosis of cell death

A method for measuring through optical means temporally resolved, optical properties, and/or phenotypes that are linked to cellular homeostasis. Those temporal measurements enable the detection of cell regulation through various channels linked to homeostasis, in order to monitor cell viability.

Method for monitoring cell viability

Apparatus and method are provided for monitoring and measuring matter and energy fluxes by use of devices able to detect refractive index changes. In one aspect, apparatus use an interference between two electromagnetic radiations in order to provide high sensitivity, enabling fluxes monitoring at the microscopic scale, by measuring phase changes or Optical Path Length (OPL) changes. In one aspect, methods are provided for monitoring and measuring the electrical activity of a biological cells, simultaneously on several cells, without use of electrodes and contrast agents.

Monitoring energy and matter fluxes by use of electromagnetic radiations

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or digital lenses (DL), specifically designed for the digital processing of wave fronts defined in amplitude and phase. DWFO are of particular interest for correcting undesired wave front deformations induced by instrumental defects or experimental errors. DWFO may be defined using a mathematical model, e.g. a polynomial function, which involves coefficients. The present invention describes automated and semi-automated procedures for calibrating or adjusting the values of these coefficients. These procedures are based on the fitting of mathematical models on reference data extracted from specific regions of a wave front called reference areas, which are characterized by the fact that specimen contributions are a priori known in reference areas. For example, reference areas can be defined in regions where flat surfaces of a specimen produce a constant phase function. The present invention describes also how DWFO can be defined by extracting reference data along one-dimensional (1D) profiles. DWFO can also be defined in order to obtain a flattened representation of non-flat area of a specimen. Several DWFO or DL can be combined, possibly in addition with procedures for calculating numerically the propagation of wave fronts. A DWFO may also be defined experimentally, e.g. by calibration procedures using reference specimens. A method for generating a DWFO by filtering in the Fourier plane is also described. All wave front sensing techniques may benefit from the present invention. The case of a wave front sensor based on digital holography, e.g. a digital holographic microscope (DHM), is described in more details. The use of DWFO improves the performance, in particular speed and precision, and the ease of use of instruments for wave front sensing. The use of DWFO results in instrumental simplifications, costs reductions, and enlarged the field of applications. The present invention defines a new technique for imaging and metrology with a large field of applications in material and life sciences, for research and industrial applications.

Wave front sensing method and apparatus

The present invention is related to a method for performing digital holographic imaging (DHI), said DHI being characterized by the fact that images of a sample are obtained by applying numerical means to reconstruct holograms of the sample. In the method of the invention, the sample is in a medium with controlled properties that influence the behaviour of the sample, and/or that influence the process of hologram formation. The information content of one hologram, or of a plurality of holograms, recorded with the sample in one medium, or in a plurality of medium, is used to reconstruct one image of the sample, or a plurality of images of the sample, that describe quantitatively one property of the sample, or a plurality of properties of the sample. The present invention is also related to an apparatus with which to perform said method.

Apparatus and method for digital holographic imaging

Growing community of inventors

Cheseaux-sur-Lausanne, Switzerland

Pierre Marquet