Centre National De La Recherche Scientifique

Universite Claude Bernard – Lyon1

Université Grenoble Alpes

Centre National De La Recherche Scientifique-cnrs

Université Claude Bernard Lyon I

Universite Claude Bernard De Lyon 1

Pierre G Falson

Anthony William Coleman

Frederic Huche

Antoine Leydier

François Penin

Julien Dauvergne

Ahcène Boumendjel

Cyrille Mbemba

Marine Peuchmaur

Kim Anh Nguyen

Rima Matar

Sandrine Magnard

Rachel Rima Matar Merheb

Cédric Montigny

Joëlle Paris

Atillio Di Pietro

Marie-Emmanuelle Million

Ali Koubeissi

Attilio Di Pietro

Frédéric Huché

Laurent Ettouati

Ophélie Arnaud

The present invention relates to a compound of formula (I): (I) as defined in the description. The present invention also relates to a method for extracting biological membrane-associated membrane proteins, comprising a step of bringing an aqueous solution of biological membrane-associated membrane proteins into contact with at least one compound of the invention. The present invention also relates to a method for stabilizing membrane proteins in solution in an aqueous solution, comprising a step (i) consisting in bringing an aqueous solution of a membrane protein in solution into contact with at least one compound of the invention.

Surfactant compounds-clips for extraction and stabilization in solution of membrane proteins

The present invention relates to the use and to a process involving at least one calix[n]arene derivative substituted by at least one acid functional group on the upper face and at least one aliphatic chain of variable length on the other face, as an additive for crystallization of a polar and/or positively charged molecule. The use and the process of the present invention have the advantage of enabling, facilitating and/or accelerating the crystallization of polar and/or positively charged molecules, especially of membrane proteins that are in solution or soluble, which had previously proved to be very difficult.

Additive for the crystallization of proteins, use and process

The present invention relates to a method for selectively extracting membrane proteins using at least one calixarene of formula (I). The use of calixarenes in the method according to the invention enables the selective solubilization of the membrane proteins while preserving the three-dimensional structure that is essential to the enzymatic activity thereof. 

Method for selectively extracting membrane proteins using calixarenes

The present invention relates to a system for expressing toxic proteins, to an expression vector comprising this system, to a prokaryotic cell transformed with this system, and also to a method for synthesizing a toxic protein using this expression system. The expression system of the invention is characterized in that it comprises successively, in the 5'-3&#x2032; direction, a nucleotide sequence encoding the Asp-Pro dipeptide and a nucleotide sequence encoding a toxic protein. According to a preferred embodiment of the invention, the expression system also comprises, upstream of the Asp-Pro sequence, a nucleotide sequence encoding a soluble protein. The expression system of the invention makes it possible to construct an expression vector that is useful for transforming a prokaryotic cell such as, for example in a method for synthesizing the toxic protein.

Systems for expressing toxic proteins, vectors and method of producing toxic proteins

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Annonay, France

Pierre G Falson