Bioeurope

Centre National De La Recherche Scientifique

Roussel Uclaf

Institut National De La Recherche Agronomique

Institut National Des Sciences Appliquees De Toulouse

Roussel-ulcaf

Ulice S.a.

Ulice

Sucre Recherches Et Developpements

Pierre F Monsan

Francois B Paul

Vincent P Pelenc

Jacques Biton

Jean-Marc Michel

Daniel H Auriol

Francois Mb Paul

Gerard Gellf

Magali M Remaud

Magali Remaud-Simeon

Claire Moulis

Gabrielle Potocki-Veronese

Jean-Pierre H Lamothe

Marianne G Graber-Gubert

Francedillaois Marie Paul

David Frank Perry

Joseph C Morin

Francis L Duchiron

Agustin Lopez Mungica Canales

Yves G Marchenay

The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the &#x3b1;-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of &#x3b1;-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.

Construction of new variants of dextransucrase DSR-S by genetic engineering

Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a process for

Disclosed is a process of enzymatically preparing .alpha.-glucoside esters. First, .alpha.-glucosides are produced by placing an acyclic alcohol or a mixture of acyclic alcohols having a water solubility of at least 2.7% v/v at 20.degree. C. in contact with starch, maltodextrins or maltose, in the presence of a purified enzymatic preparation having .alpha.-transglucosylation activity, wherein the enzymatic preparation is free of .beta.-glucosidase activity. Then, the .alpha.-glucosides are contacted with at least one fatty acid and a preparation having lipase activity to produce the .alpha.-glucoside esters, which may then be recovered. The preparation having .alpha.-transglucosylation activity may originate from a fungus such as Talaromyces duponti, Aspergillus niger, Aspergillus oryzae or Aspergillus batatae.

Methods for the production of esters of .alpha.-glucosides and uses

Process for the enzymatic synthesis of alkyl esters of peptides and

Cosmetic compositions containing oligosaccharides

The present invention concerns the use of fructosyl oligosaccharides as low-calorie sweeteners, and the foods, dietetic products and drinks containing them.

Low calorie sweetener

Method for the production of .alpha.(1.fwdarw.2) oligodextrans using

Low-calorie sweeteners of fructosyl oligosaccharides and the food or

The invention relates to the preparation of water-soluble L-tyrosine derivatives having the formula ##STR1## where R.sub.1 is a L-malyl, L-lactyl, L-glutamyl or L-aspartyl group, R.sub.2 is a --OH, --OM, methoxy, ethoxy or amino group and M is an alkaline metal, by enzymatic condensation of an acid selected among L-malic, L-lactic, L-glutamic and L-aspartic acids, with a L-tyrosine derivatives selected among the ethyl and methyl esters and the amide of L-tyrosine. The enzyme is obtained from a culture of Micrococcus casealyticus. The compounds where R.sub.1 is a L-malyl or L-lactyl group are new. Said L-tyrosine derivatives are useful in cosmetic and pharmaceutical compositions, such as tanning and sun compositions.

Water-soluble L-tyrosine derivatives and a process for preparing

The invention relates to biotechnology. It relates, in particular, to cultures of Klebsiella sp. microorganisms possessing the characteristics of the strain BEC 441 deposited under No. I-714 at the Collection Nationale de Cultures de Micro-organismes (National Collection of Microorganism Cultures) of the Institut Pasteur, as well as strains obtained from the strain BEC 441 by mutation, these strains being capable of producing polysaccharides rich in rhamnose by fermentation in a nutrient medium containing assimilable sources of carbon and of nitrogen and inorganic substances. Application for the production of a mixture of monosaccharides having a high content of rhamnose.

Culture of a microorgansim of the genus klebsiella sp., having a high

The invention relates to the field of biotechnology. It relates to a process for preparing a syrup, the dry extract of which includes a high content of isomaltose, which comprises treating an aqueous solution of sucrose with a mixture of the enzymes dextransucrase and dextranase at a temperature of 0.degree. to 50.degree. C. and at a pH in the range from 4.5 to 7, so as to obtain an aqueous syrup containing fructose, isomaltose, glucose and, possibly, isomaltotriose as the main constituents. The syrup obtained is a useful intermediate for the synthesis, in particular, of isomaltitol.

Process for the enzymatic preparation from sucrose of a mixture of

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Blagnac, France

Pierre F Monsan