E.i. Dupont De Nemours and Company

Danisco US Inc.

Pierre E Rouviere

Qiong Cheng

Stephen R Fahnestock

Tanja Maria Gruber

Patricia C Brzostowicz

Scott D Cunningham

Linda Jane Solomon

Hong Peng Wang

Luan Tao

Wonchul Suh

Kari A Fosser

Robert DiCosimo

Mark Scott Payne

Stephen K Picataggio

James M Odom

Mattheos Koffas

Vasantha Nagarajan

Steven Dale Ittel

John P O'Brien

Michael G Bramucci

Jean-Francois Tomb

Albert W Alsop

Stuart M Thomas

Kelley C Norton

Kristy N Kostichka

Linda Jane DeCarolis

Laura Anne Bedzyk

Andreas Schenzle

Dana M Walters

Rainer Russ

Lisa Diane Reiss

Anthony John Kinney

Douglas Robert Anton

Daniel P O'Keefe

Sharon Loretta Haynie

Eberhard Schneider

Peter Wagner

Dana M Walters Pollak

Gregor Schurmann

Kristin Ruebling-Jass

Daniel P Okeefe

Qi Chen

Hao Ni

Annapurna Sachan

Ritu Bhalla

Neeraj Pandey

Deana DiCosimo

Edward S Miller

Lisa A Butterick

Hong Wang

Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.

Recombinant host cells having an increase in buoyant density

Compositions and methods are provided using fusion peptides comprising at least one multi-functional solubility tag having an effective number of cross-linkable cysteines residues. The multi-functional peptidic solubility tags facilitate efficient fusion peptide production, easier downstream processing of the fusion peptide, and provide functional surface properties when coupled to a target material while the cross-linkable cysteines provide enhanced durability when binding the fusion peptide to a target material.

Cysteine cross-linked structural peptides

Compositions and methods using fusion peptides comprising multi-functional solubility tags are provided. The multi-functional peptidic solubility tags facilitates more efficient fusion peptide production, easier downstream processing of the fusion peptide, and may be used to provide functional surface properties when coupled to a target material.

Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications

Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.

Targeted perhydrolases

An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.

Acid-cleavable linkers exhibiting altered rates of acid hydrolysis

Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.

Enzymatic peracid generation for use in oral care products

The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.

Recombinant peptide production using a cross-linkable solubility tag

Oral care compositions, oral care systems, oral surface-binding peptides, and a method for applying particles to an oral surface are provided. The oral care system comprises at least one peptidic component comprising a first binding element having affinity for an oral surface and a second binding element having affinity for a ligand property of a particle.

Peptide compositions for oral care systems

Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.

Solubility tags for the expression and purification of bioactive peptides

Combinatorially generated peptides are provided that have binding affinity for calcium carbonates. The peptides may be used to deliver benefit agents to various calcium carbonate surfaces.

Peptides for binding calcium carbonates and methods of use

Combinatorially generated peptides are provided that have binding affinity for clay. The peptides may be used to deliver benefit agents to various clay surfaces.

Clay-binding peptides and methods of use

Growing community of inventors

Wilmington, DE, United States of America

Pierre E Rouviere