Commissariat a L'energie Atomique Et Aux Energies Alternatives

Horiba Abx Sas

Iprasense Sas

Horiba Abx Sas / Horiba Ltd

Pierre Blandin

Lionel Herve

Olivier Cioni

Cedric Allier

Etienne Quesnel

Benoit Racine

Damien Isebe

Anais Ali-Cherif

Aurelien Daynes

Estelle Gremion

Anais Ali Cherif

Thomas Bordy

Pierre Joly

Cédric Allier

Geoffrey Esteban

Sebastien Raimbault

Jean-Marc Dinten

Device () for characterizing a substance () capable of emitting a photoluminescence radiation (Rp) in a first spectral range, the device () comprising:

Compact device for characterizing a photoluminescent substance

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest.

Method for characterising a particle on the basis of a hologram

A device for exciting objects with an excitation radiation and for detecting a photoluminescence radiation emitted by the objects after the absorption of the excitation radiation. The device includes a wall in contact with the objects, an organic light-emitting diode for emitting the excitation radiation and transparent to the photoluminescence radiation, an optical resonator tuned to the wavelength of the photoluminescence radiation and located on the side of the organic light-emitting diode opposite to the wall, and at least one sensor of the photoluminescence radiation arranged on the side of the optical resonator opposite to the organic light-emitting diode.

Excitation and photoluminescence detection device

The invention is a method for estimating a representative volume of particles of interest () immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (), the light source emitting an incident light wave () propagating towards the sample () along a propagation axis (Z); b) acquiring, by means of an image sensor (), an image (I) of the sample (), formed in a detection plane (P), the sample being arranged between the light source () and the image sensor (), each image being representative of a light wave () referred to as an exposure light wave, to which the image sensor () is exposed under the effect of illumination; c) using the image of the sample (I), acquired during step b), and a holographic propagation operator, to calculate a complex expression (A (x, y, z)) of the exposure light wave () in different positions relative to the detection plane; the method comprising a step of estimating the representative volume (AA) of the particles of interest () depending on the complex expressions calculated during step c).

Optical method for estimating a representative volume of particles present in a sample based on a size of each region of interest

A method for observing a sample by lensless imaging, in which a sample is positioned between a laser diode and an image sensor, the laser diode being supplied with a supply current whose intensity is less than or equal to a critical value. This critical intensity is determined during preliminary operations, during which the intensity is initially greater than a laser threshold of the diode. By observing the image formed at the image sensor, the intensity is decreased until an attenuation of the interference images on the formed image is observed, the critical intensity corresponding to the intensity at which this attenuation is optimum.

Method for observing a sample by lensless imaging

The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Method for counting particles in a sample by means of lensless imaging

A device and method for forming an image of a sample includes illuminating the sample with a light source; acquiring a plurality of images of the sample using an image sensor, the sample being placed between the light source and the image sensor, no magnifying optics being placed between the sample and the image sensor, the image sensor lying in a detection plane, the image sensor being moved with respect to the sample between two respective acquisitions, such that each acquired image is respectively associated with a position of the image sensor in the detection plane, each position being different from the next; and forming an image, called the high-resolution image, from the images thus acquired.

Method for forming a high resolution image by lensless imaging

A method for holographic characterization of a particle contained in a sample, based on an image, or hologram, of the sample obtained by an image sensor when the sample is illuminated by a light source. The hologram is the subject of a holographic reconstruction, to obtain a reference complex image, representative of the light wave transmitted by the sample in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, to obtain a plurality of secondary complex images, from which a profile is determined describing the change in an optical feature of the light wave transmuted by the sample along the axis of propagation of the light wave.

Holographic method for characterizing a particle in a sample

A method for identifying a particle contained in a sample, including illuminating the sample using a light source, the light source producing an incident light wave propagating toward the sample, then acquiring, using a matrix-array photodetector, an image of the sample, the sample being placed between the light source and the photodetector such that the matrix-array photodetector is exposed to a light wave that is the result of interference between the incident light wave and a diffraction wave produced by each particle. The method further includes applying a numerical reconstruction algorithm to the image acquired by the photodetector, to estimate a characteristic quantity of the light wave reaching the detector, at a plurality of distances from the detector. The variation in the characteristic quantity as a function of distance allows the particle to be identified.

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Coublevie, France

Pierre Blandin