Saint-gobain Performance Plastics Corporation

National Institutes of Health, a Component of the US Dept. of Health & Human Services

American Fluoroseal Corporation

Phillippe Jean Broussard

Herbert Myers Cullis

Sarah Louise Clark

Richard W Childs

Natasha Anna Lundgren

Jeffrey Ellis Miripol

Herb Cullis

Sumithira Vasu

Kevin Douglas Clark

Edouard Civel

Eric Kelsey Harting

Jacob Andrews

Robert G Pleydon

Natasha Anna Boghosian

A cell culture container is provided, comprising membrane bound compartments for growing and harvesting monocytes that allows replenishing and removal of feed media without the loss or death of cells.

System for culture of cells in a controlled environment

The disclosure relates generally to a cell culture apparatus and a cell culture method. One aspect of the disclosure provides a perfusion cell culture bag comprising one or more polymer films, having edges bonded together to form edges around an undivided interior compartment of the bag; first and second ports each formed in an exterior surface of the bag; first and second liquid-permeable tubes each extending into and in fluid communication with the interior compartment of the bag and operatively coupled to a respective port; and a third port formed in an exterior surface of the bag, the third port being in fluid communication with the interior compartment of the bag; wherein the first tube comprises a first inner support structure defining a central lumen of the tube and a first outer filter layer surrounding the first inner support structure.

Cell culture system

A container includes an inner bag, where the interior of the inner bag includes a sterile environment for storing a material, at least one access port configured to provide fluid access to the interior of the inner bag, an overwrap bag, where the interior of the overwrap bag includes a sterile environment for storing the material and where the inner bag is enclosed within the interior of the overwrap bag, and an overwrap access port configured to provide fluid access to the interior of the overwrap bag. The material can include a biomaterial. Each of the inner bag, the overwrap bag, the at least one access port, and the overwrap access port can include a fluoropolymer such as fluoroethylenepropylene (FEP).

Cryopreservation container

Methods and apparatus for selectively accessing a portion of a sterile cryopreserved sample are disclosed. The apparatus may include a container configured to receive the cryopreserved sample and having a first portion and a second portion, a heat sink chamber surrounding the first portion of the container, and a heat source adjacent to the second portion of the container. The chamber may be configured to maintain a non-accessed portion of the sample in a cryopreserved state. The heat source may be configured to separating an accessed portion of the sample from the non-accessed portion of the sample while maintaining the viability of the accessed portion while the non-accessed portion is maintained in the cryopreserved state.

Selective access to cryopreserved samples

Growing community of inventors

Finksburg, MD, United States of America

Phillippe Jean Broussard