Indigo Ag, Inc.

Ait Austrian Institute of Technology Gmbh

Axcella Health Inc.

Indigo Agriculture, Inc.

Generation Bio Co.

Phillip Samayoa

Geoffrey Von Maltzahn

Gerardo Vicente Toledo

Slavica Djonovic

Luis Miguel Marquez

Richard Bailey Flavell

Craig Sadowski

David Morris Johnston

Yves Alain Millet

Jeffrey Lyford

Jonathan W Leff

Birgit Mitter

Angela Sessitsch

Muhammad Naveed

Teresa Berninger

Stephane Compant

Nathaniel Silver

Karen V Ambrose

Xuecheng Zhang

David Arthur Berry

Michael Hamill

Brett Adam Boghigian

Shaila Rahman

John Kramarczyk

Michael Doherty

Douglas Anthony Kerr

David Young

Subhayu Basu

Ying-Ja Chen

Kathryn Heard

Caitlyn Harvey

Vimal Vaidya

Jameson Rogers

David Weisman

Trudi A Gulick

Debra Klatte

Stephanie M Liva

Matthew Chiocco

Nathan A Billings

Karen Ambrose

David R Weisman

The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding PAH protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of PAH protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of PAH protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing PAH protein. Such PAH protein can be expressed for treating disease, e.g., Phenylketonuria (PKU).

Non-viral DNA vectors and uses thereof for expressing phenylalanine hydroxylase (PAH) therapeutics

The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding FVIII protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of FVIII protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of FVIII protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing FVIII protein. Such FVIII protein can be expressed for treating disease, e.g., hemophilia A.

Non-viral DNA vectors and uses thereof for expressing FVIII therapeutics

The present invention relates to methods of scalably producing microorganisms by propagating them within plant tissues and introducing them into agricultural seeds to improve their shelf-life during long-term storage, to produce substances of interest, and to create libraries of microbes.

Method for propagating microorganisms within plant bioreactors and stably storing microorganisms within agricultural seeds

Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.

Endophytes, associated compositions, and methods of use

Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

Nutritive polypeptides and formulations thereof, and methods of production and use thereof

This application relates to methods and materials for providing a benefit to a seed, seedling or plant by producing seeds that are internally colonized with endophytes. Beneficial endophytes with particular characteristics are provided. These may be used in the methods described to provide a benefit to a seed, seedling or plant.

Plants containing beneficial endophytes

Materials and methods for improving plant traits and for providing plant fitness benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.

Seed endophytes across cultivars and species, associated compositions, and methods of use thereof

Endophytes, associated compositions, and methods of use thereof

Growing community of inventors

Cambridge, MA, United States of America

Phillip Samayoa