Renovo Limited

Phillip Mellors

Mark William James Ferguson

Sharon O'Kane

Hugh Gerard Laverty

Nick Occleston

Emma Atkinson

Simon Higginbottom

The invention provides TGF-&#x3b2;3s, or fragments or derivatives thereof, wherein the alpha-helix-forming domain between amino acid residues (58) and (67) of full-length wild type TGF-&#x3b2;3 comprises at least one alpha-helix-stabilizing substitution. The invention also provides TGF-&#x3b2;3s, or fragments or derivatives thereof, wherein the Glycine residue at position (63) of full-length wild type TGF-&#x3b2;3 is replaced with Proline. Further still, the invention provides TGF-&#x3b2;3s, or fragments or derivatives thereof, comprising a substitution of the Glutamic acid residue at position (12) of full-length wild type TGF-&#x3b2;3 and/or the Arginine residue at position (52) of full-length wild type TGF-&#x3b2;3. The invention also provides medicaments and methods of treatment using such TGF-&#x3b2;3s.

TGF-&#x3b2;3 mutants

There is provided the use of monomeric TGF-&#x3b2;s, or there fragments or derivatives, as medicaments. These medicaments preferably comprise monomeric TGF-&#x3b2;3, or fragments or derivatives thereof. The medicaments provided may be used in the acceleration of wounding and/or the inhibition of scarring, in the promotion of epithelial regeneration, or in the prevention and/or treatment of fibrotic disorders.

Medicaments and proteins based on TGF-&#x3b2; monomers for the treatment of wounds

The present invention concerns a method for folding a Transforming Growth Factor Beta, or a functional analogue thereof, into a dimeric, biologically active form. The method involves adding solubilized, unfolded monomeric growth factor to a solution containing 2-(cyclohexylamino)-ethanesulfonic acid (CHES) or a functional analogue thereof and a low molecular weight sulfhydryl/disulfide redox system. The solution is then incubated under conditions suitable for generating dimeric biologically active Transforming Growth Factor Beta.

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Manchester, United Kingdom

Phillip Mellors