University of California

Phillip Kyriakakis

Todd Prentice Coleman

Marianne Catanho

Methods, systems, and devices are disclosed for in vivo production or biosynthesis of metabolites in foreign cells using the combination of (i) one or more ferredoxin dependent enzyme(s) and (ii) a ferredoxin (Fd)/ferredoxin-NADPreductase (FNR) system. The ferredoxin dependent enzymes and the Fd/FNR system are from the same species or from a different but matching species.

System and method for biosynthesis

Disclosed herein are methods of assembling nucleic acid fragments, such as non overlapping nucleic acid fragments, in an orientation-specific, sequence-independent way. The methods entail annealing one or more nucleic acid fragments to be assembled onto a solid phase via matching barcodes having sequences complementary to the solid phase oligos, ligating the nucleic acid fragments by end-to-end ligation, and recovering the ligated nucleic acid from the solid phase.

Growing community of inventors

San Diego, CA, United States of America

Phillip Kyriakakis