Boston University

Phillip J Stone

Carl Franzblau

Julianne Bryan-Rhadfi

A method for determining the quantity of a connective tissue or muscle tissue breakdown product in a body fluid from an animal includes steps of providing a standard comprising the breakdown product having a label, the standard having a known specific activity, combining the standard and a sample of the body fluid, removing from the combined standard and sample a purified breakdown product fraction containing labelled breakdown product from the standard together with breakdown product from the sample, and measuring the specific radioactivity of the fraction as a measure of the quantity of the breakdown product in the sample. Also, methods for assessing, in a body fluid from an animal, the condition of a selected connective tissue or a muscle tissue in an animal, and for assessing a disease process that includes destruction of a specified connective tissue component or muscle tissue, and for assessing the efficacy of a therapy for treatment of such a disease process, include the steps of the method for determining the quantity of a tissue breakdown product.

Measuring tissue breakdown products in body fluids

A method for determining a connective tissue breakdown product in a body fluid from an animal includes steps of providing a standard comprising the breakdown product having a radioactive label, the standard having a known specific radioactivity, combining the standard and a sample of the body fluid, removing from the combined standard and sample a purified breakdown product fraction containing labelled breakdown product from the standard together with breakdown product from the sample, and measuring the specific radioactivity of the fraction as a measure of the quantity of the breakdown product in the sample. Also, methods for assessing a condition of a selected connective tissue in an animal in vivo, and for assessing in vivo a disease process that includes destruction of a specified connective tissue component, and for assessing in vivo the efficacy of a therapy for treatment of such a disease process, include the steps of the method for determining a connective tissue breakdown product.

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Chestnut Hill, MA, United States of America

Phillip J Stone