United States of America, as Represented by the Secretary of Agriculture

Phillip E Gaush

Norman W Schaad

Meric Ozakman

, the causal agent of bacterial brown rot of potato, is often carried latently in seed potato tubers. Primers and a probe were designed for a real-time BIO-PCR assay technique for detecting potato tubers latently infected with. Using naturally infected potato tubers, as few as 20 cells/ml extract could be detected. Two of 14 naturally infected potato tubers with no disease symptoms were positive by the newly described real-time BIO-PCR (pre-enrichment on agar or in liquid medium) assay but not by direct real-time PCR.

Growing community of inventors

Charles Town, WV, United States of America

Phillip E Gaush