Centre National De La Recherche Scientifique

Other

Institut Curie

Institut Gustave Roussy

Université Pierre Et Marie Curie

Philippe Vielh

Genevieve Almouzni

Fabrice Andre

Stefan Michiels

Suzette Delaloge

Marc Spielmann

Philippe Dessen

Sophie E Polo

Stamatios E Theocharis

Khuong Truong

Bernard Malfoy

Claire Bourgeois

Marie-No&euml;lle Guilly

Bernard Dutrillaux

The invention relates to the use of a multiplicity of polynucleotide probe sets, the multiplicity of polynucleotide probe sets consisting in a combination of pools of polynucleotide probe sets, each polynucleotide probe set containing at least one polynucleotide probe chosen among a library of nucleic acid sequences, the polynucleotide probes involved in the combination of pools of polynucleotide probe sets of the multiplicity of polynucleotide probe sets being such that each polynucleotide probe specifically hybridizes with one gene, and/or at least one of its variants when present, for determining the variation of expression at least 12 genes, and their variants when present, in order to diagnose the benign or malignant state of a breast tumor.

Molecular in vitro diagnosis of breast cancer

The invention relates to a method for assessing the proliferative state of cells in a human or non human biological sample, comprising using Chromatin Assembly Factor-1 (CAF-1 in short) subunits as proliferation markers. Application for cancer prognosis or diagnosis and monitoring tumor response in therapy.

Proliferation markers in clinical practice and their use for cancer prognosis or diagnosis

The invention concerns a method for detecting intrachromosome imbalance in interphase nuclei, characterized in that it consists in: (a) hybridizing in situ the chromosome suspected to be affected by said imbalance using two probes marked with different fluorochromes and specific of said chromosome long arm and short arm respectively; (b) measuring the intensity of the fluorescence emitted in said nuclei with different emission wavelengths corresponding to each of the two fluorochromes; (c) calculating the ratio (Ra) between the two measured values of fluorescence intensity; and (d) comparing the resulting ratio value with at least one reference value. The invention also concerns a kit for implementing said method and uses of said kit and said method, in particular for detecting cancer cells in a biological sample and for diagnosing cancer pathologies.

Method and kit for detecting intrachromosome imbalance in interphase nuclei and their applications

Growing community of inventors

Paris, France

Philippe Vielh