Metabolic Explorer

E.i. Dupont De Nemours and Company

Centre National De La Recherche Scientifique

Danisco US Inc.

Institut National De La Recherche Agronomique

Genencor International, Inc.

Institut National Des Sciences Appliquees

Institut National De La Recherche

Evonik Degussa Gmbh

Institut National Des Sciences Appliquees De Toulouse

Metabolic Exployer

Danisco US In.

Institut National Des Sciences

Evonik Operations Gmbh

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Philippe Soucaille

Rainer Figge

Fernando Valle

Laurence Dumon-Seignovert

Gwénaëlle Bestel-Corre

François Voelker

Christian Croux

Marguerite A Cervin

Donald Eugene Trimbur

Michel Chateau

Wanda Dischert

Cédric Boisart

Mark Scott Payne

Stephen K Picataggio

Ramesh V Nair

Céline Raynaud

Isabelle Meynial-Salles

Amy Kuang-Hsu

Patricia Sarcabal

Gregory Marshall Whited

Fabien Lux

Benjamin Gonzalez

Gwenaëlle Corre

Zhen Chen

Jean-Yves Dubois

Perrine Vasseur

Guillaume Barbier

An-Ping Zeng

Olivier Zink

Pascale Aliprandi

Fernado Valle

Isabelle Meynial Salles

Feng Geng

Benjamin Percheron

Ngoc-Phuong-Thao Nguyen

Gwénaëlle Bestel Corre

Emilie Navarro

Antoine Riviere

Céline Foulquier

Amy K Hsu

Francois Voelker

Gwenaelle Bestel Corre

Celine Foulquier

Celine Raynaud

Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.

Increasing methionine yield

The present invention relates to a recombinant microorganism capable of producing 2,4-dihydroxybutyrate, which is characterized by an increased cellular export, and preferably by a decreased cellular import, of said 2,4 DHB. The invention also relates to a method for the optimized production of 2,4-dihydroxybutyrate by culturing said microorganism in a fermentation medium and recovering 2,4-DHB from said medium.

Modified microorganism for the optimized production of 2,4-dihydroxybutyrate with enhanced 2,4-dihydroxybutyrate efflux

The present invention relates to a genetically modified microorganism for the fermentative conversion of levulinic acid into propionyl-CoA and acetyl-CoA, and to a fermentation process for performing said conversion.

Microorganism modified for the assimilation of levulinic acid

The present invention relates to methods and recombinant microorganisms useful for the production of glycolic acid and/or glyoxylic acid. The methods of the invention involve either only one step of fermentation of the modified microorganisms of the invention or one step of fermentation of the modified microorganisms of the invention and one step of either biological or chemical conversion of the glycolic acid or of the glyoxylic acid, the microorganism of the invention over expressing a phosphoketolase gene.

Methods and microorganisms for the production of glycolic acid and/or glyoxylic acid

The present invention relates to a novel polypeptide having the enzymatic activity of reduction of NADP+ using electrons from reduced ferredoxin (Ferredoxin-NADP+ reductase activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. The invention relates to the modulation of the Ferredoxin NADP+ reductase activity in a microorganism by varying the expression level of the polynucleotide coding for such polypeptide. The invention also relates to the production of commodity chemicals, especially ethanol, n-butanol, 1,3-propanediol, 1,2-propanediol, isopropanol and acetone by fermenting microorganisms wherein their Ferredoxin NADP+ reductase activity is modulated.

Polypeptide having ferredoxin-NADP+ reductase activity, polynucleotide encoding the same and uses thereof

The present invention relates to a recombinant microorganism useful for the production of 1,2-propanediol and process for the preparation of 1,2-propanediol. The microorganism of the invention is modified in a way that the 1,2-propanediol production is improved by enhancing NADPH dependent HAR activity.

Microorganism and method for the production of 1.2-propanediol based on NADPH dependent acetol reductase and improved NADPH supply

The present invention relates to a new strain ofmodified to be unable to produce hydrogen and its use for the continuous production of bulk chemicals such as lactate, 1,3-propanediol, ethanol, butanol, isobutanol, 1,3-butanediol, acetate, acetone, isopropanol, 3-hydroxy-3-methylbutyrate and isobutene at high yield.

strains unable to produce hydrogen and useful for the continuous production of chemicals and fuels

The present invention relates to a genetically modified microorganism for the production of 2,4-dihydroxybutyrate, by metabolic transformation of xylose via the 1,2,4-butanetriol intermediate. The invention also relates to a method for the production of 2,4-dihydroxybutyrate by culturing said genetically modified microorganism in a fermentation medium and recovering 2,4-DHB from said medium.

Modified microorganism for the optimized production of 2,4-dihydroxybutyrate

The present invention concerns a method for the production of derivatives of 4-hydroxy-2-ketobutyrate chosen among 1,3-propanediol or 2,4-dihydroxybutyrate by culturing a genetically modified microorganism for the production of the desired derivative of 4-hydroxy-2-ketobutyrate, the microorganism further comprising a gene coding for a mutant glutamate dehydrogenase converting by deamination L-homoserine into 4-hydroxy-2-ketobutyrate. The invention also concerns said genetically modified microorganism.

Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate

The present invention is relative to a method for producing 1,2-propanediol by fermentation, comprising: cultivating a microorganism producing 1,2-propanediol in an appropriate medium comprising a source of sucrose, and recovering the 1,2-propanediol being produced, wherein the microorganism is able to utilize sucrose as sole carbon source for the production of 1,2-propanediol. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass, and is in particular sugar cane juice.

Use of sucrose as substrate for fermentative production of 1,2-propanediol

The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.

Method of creating a library of bacterial clones with varying levels of gene expression

A method of producing methionine, derivatives or precursors thereof includes culturing a modified microorganism in a culture medium comprising a source of carbon and a source of sulfur; and recovering methionine from the culture medium, wherein said modified microorganism has an increased expression of cysE gene encoding serine acetyltransferase, metH gene encoding methionine synthase and metF gene encoding 5,10-methylenetetrahydrofolate reductase compared to expression of the cysE, metH and metF genes in an unmodified microorganism.

Methods for producing methionine by culturing a microorganism modified to enhance production of cysteine

The present invention relates to use of inducible promoters in the production of glycolic acid by fermentation. The present invention concerns a method for the production of glycolic acid in a fermentative process comprising the following steps: The invention also concerned the modified microorganism used in the method of glycolic acid production.

Method for producing glycolic acid using an inducible promoter

The present invention concerns a new method for the biological preparation of a diol comprising culturing a microorganism genetically modified for the bioproduction of an aliphatic diol, wherein the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol, and wherein the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite. The invention also concerns a modified microorganism for the production of an aliphatic diol.

Method for the preparation of diols

The present invention concerns a modified microorganism with an increased methylglyoxal reductase activity, and its use for the preparation of 1,2-propanediol and/or acetol. In particular this increased methylglyoxal reductase activity is obtained by increasing the expression of specific genes from microorganisms. This invention is also related to a method for producing 1,2-propanediol and/or acetol by fermentation of a microorganism having an increased methylglyoxal reductase activity.

Microorganisms and methods for production of 1,2-propanediol and acetol

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Deyme, France

Philippe Soucaille