Commissariat a L'energie Atomique

Philippe Pradelles

Jacques Grassi

Eric Ezan

Marie-Astrid Sagot

The present invention relates to a method for detecting and/or measuring the concentration of fluoride (F) or hydrogen fluoride (HF) in a sample, comprising the steps consisting of bringing said sample, in aqueous solution, into contact with a silylated organic compound in order to obtain a measurement solution, with said silylated organic compound being desilylated when it is in the presence of hydrofluoric acid or a fluoride, with the silylated organic compound and the desilylated organic compound being able to be detected and/or measured separately from each other; and detecting and/or measuring, in said measurement solution, the appearance of the desilylated against compound or the disappearance of the silylated organic compound, which takes place if fluoride or hydrogen fluoride is present in the sample. The method enables the presence of hydrogen fluoride or of fluorine to be detected very easily and expediently at concentrations of 10l of HF/10l of air (10 ppb) or else of 0.5 to 1 &#x3bc;g/ml of HF in solution. The kit of the present invention comprises the components which are required for implementing this method. The method of the invention makes it possible to detect fluorine at concentrations of the order of 0.001 &#x3bc;g/ml.

Method for the detection of fluoride or hydrogen fluoride and detection kit

The invention relates to a process for the immunometric determination of an antigen or hapten. According to this process, contacting takes place (FIG. 1A) between the antigen or hapten (3) to be determined and the antibodies (2) fixed to a solid phase in order to immunologically bond the antigen or hapten with the antibody. This is followed (FIG. 1B) by immobilizing the antigen or hapten (3) by a covalent bond (4) to the solid phase (1) whilst releasing its epitope (FIG. 1C). This is followed by the contacting thereof (FIG. 1D) with labelled antibodies (5) and determination takes place (FIG. 1E) of the quantity of fixed labelled antibodies in order to deduce therefrom the initial hapten or antigen concentration. As a result of this stage of immobilizing and releasing the epitope, a high sensitivity is obtained using a single antibody.

Immunometric determination of an antigen or hapten

Compound labelled by the acetyl cholinesterase of Electrophorus electricus, its preparation process and its use in enzymoimmunology. This compound is constituted by a molecule chosen from among the antigens, haptens and antibodies, bonded by a covalent bond to an enzyme formed by the acetyl cholinesterase of Electrophorus electricus (electric eel). For example, the molecule is the substance P or a prostaglandin and the compound can be used for the enzymoimmunological determination of these molecules.

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Longjumeau, France

Philippe Pradelles