Laboratoire Francais Du Fractionnement Et Des Biotechnologies

Laboratoire Francais Du Fractionnement Et Des Biotechnologies Societe Anonyme

Laboratoiore Francais Du Fractionnement Et Des Biotechnologies

Philippe Paolantonacci

Abdessatar Chtourou

Roland Schmitthaeusler

Frédéric Dhainaut

Monique Ollivier

Jacky Lirochon

Abdessatar Sami Chtourou

Frederic Dhainaut

The invention relates to an affinity chromatography matrix, as a gel, comprising polymeric particles on which at least one oligosaccharide corresponding to a blood group A epitope and/or blood group B is grafted, via a spacer, characterized in that the density of oligosaccharides is comprised between 0.2 and 0.7 mg/ml of matrix. The invention also relates to the uses of this matrix for preparing concentrates of immunoglobulins for therapeutic use.

Affinity chromatography matrix

The present invention relates to an immunoglobulin G concentrate for therapeutic use, in which the respective contents of anti-A and anti-B antibodies are in accordance with a negative result in the in vitro indirect Coombs test. This IgG concentrate also has a polyreactive IgG content of between 0.01% and 0.1%, in particular between 0.07% and 0.1%, relative to the total content of IgG.

Immunoglobulin G (IgG) concentrate depleted of anti-A and anti-B antibodies and of polyreactive IgGs

The invention concerns a method for preparing human immunoglobulin concentrates for therapeutic use, from plasma or a plasma fraction. The method comprises pre-purification and a single anion-exchange chromatography carried out at alkaline pH, thereby enabling the immunoglobulins to be retained on the chromatographic support and fractionated. The method enables to obtain IgG, IgA and IgM concentrates.

Method for preparing human immunoglobulin concentrates for therapeutic use

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Gif sur Yvette, France

Philippe Paolantonacci