Biomerieux, Inc.

Gen-probe, Inc.

Biomerieux Sa

Other

Blomorloux S.a.

Philippe L Cleuziat

Steven T Brentano

Markus T Jucker

Francisco D Delgado

Pierre Levasseur

Francois Mallet

Francoise Guillou-Bonnici

Bruno Colin

Marc Rodrigue

Patrick Broyer

Sandra Incardona

Bernard F Mandrand

Claude Mabilat

Jean-Pierre Bernard Gayral

Corinne Jay

Cecile Jaravel

William McAllister

Lyse Santoro

Francedillaois Mallet

C&eacute;cile Paris

Jeannine Robert-Baudouy

Abalo Awade

Francedillaoise Guillou-Bonnici

Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genusare disclosed. Kits including such oligonucleotides are disclosed. Methods of detectingspecies using the oligonucleotides in in vitro nucleic acid amplification are disclosed.

Nucleic acid amplification and detection of mycobacterium species

A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.

Method for cellular lysis of prokaryotes or eukaryotes or simultaneous cellular lysis of prokaryotes and eukaryotes

A method of detecting rpoB sequences ofpresent in a biological sample that includes steps of amplifying therpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences ofin a sample are disclosed.

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A method of detecting rpoB sequences ofpresent in a biological sample that includes steps of amplifying therpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences using multiple probes that provide sequence information by their specific hybridization to portions of the amplified nucleic acid. Compositions for amplifying and detecting in vitro the rpoB sequences ofin a sample are disclosed.

Detection of rpoB sequences of Mycobacterium tuberculosis

An apparatus including at least one sonotrode ( ) designed to generate ultrasound of variable power in at least one biological sample ( ) containing cells to be lysed, the sample ( ) being contained in at least one receptacle ( or ) such that the sonotrode ( ) is in direct contact with the receptacle(s) ( ). Also disclosed is a method for using ultrasound to lyse a biological sample ( ) contained in a receptacle ( ), which includes placing the receptacle ( ) in direct contact with the sonotrode ( ), and activating the sonotrode ( ) for long enough to lyse the cells in the sample ( ) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.

Method and apparatus for ultrasonic lysis of biological cells

Methods of detecting Mycobacterium species using oligonucleotides to amplify in vitro 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species in the genus Mycobacterium are disclosed. Amplification oligonucleotides for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed. Kits containing oligonucleotides useful for in vitro amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences from many Mycobacterium species are disclosed.

The invention concerns a device for lysis ( ) of micro-organism to release at least one intracellular biological constituent, comprising a container ( ) wherein are present a biological sample, in liquid medium, containing the micro-organism to be lyzed, and a material in the form of particles, relatively hard and inert with respect to the sample. The invention also concerns a grinding method. The material in the form of particles comprises at least two types of grinding means into different dimensions: at least large dimension magnetic means ( ) automatically controlled by a magnetic field; and at least small dimension means ( ) actuated by the large dimension grinding means ( ). The invention is useful for separating biological constituents.

Device and method for the lysis of micro-organisms

The extension of primers capable of hybridizing to the nucleic acid of interest results in a process for generating single-stranded polynucleotides. The process includes contacting the nucleic acid of interest under conditions allowing extension of the primers with a first primer and a second primer capable of hybridizing to a first region and a second region, respectively, of the nucleic acid, wherein the second region overlaps with the first region.

Method for nucleic acid amplification by transcription using displacement, and reagents and kit therefor

Single-use analysis card comprising a liquid flow duct

An oligonucleotide is intended to be used as a promoter non-template strand in the transcription of a sequence of a nucleotide target in the presence of a phage RNA polymerase. The phage RNA polymerase has specific natural promoters containing a consensus sequence from at least position -17 to position -1. The oligonucleotide contains a core sequence flanked at at least one of its ends by a nucleotide sequence capable of hybridization with a sequence of the target. The core sequence contains a sequence of 6 to 9 consecutive nucleotides from the region -12 to -4 of the non-template strand of the specific promoter, or a sufficiently homologous sequence to enable the functionality of the RNA polymerase to be retained. One flanking sequence is complementary to a first region of the target, and a second flanking sequence, when present, is complementary to a second region of the target, the first and second regions being separated on the target by a sequence having a number of nucleotides equal to the number of nucleotides in the core sequence. The number of nucleotides in the flanking region, or the sum of the number of nucleotides in the flanking regions, is at least sufficiently high for the nucleotide to be able to hybridize with the target at the temperature of use of the RNA polymerase. Such an oligonucleotide enables transcription to be initiated at a site of the target which is not normally a transcription start site for the RNA polymerase.

Modified promoter for RNA polymerase, its preparation and its

Oligonucleotide comprising, from 5' to 3', a first portion comprising an upstream segment and a downstream segment forming a stem-loop structure, and a second portion not capable of self-pairing, in which the first portion comprises at least one blocking agent, capable of blocking the replication, by a polymerase, of the said oligonucleotide, such that at least one portion of the downstream segment is replicated and such that the upstream segment is replicated neither totally nor partially. This oligonucleotide can be used as primer in a process for the cyclic amplification of a target sequence of a nucleic acid.

Oligonucleotide which can be used as primer in a method of amplification

Method for nucleic acid amplification by transcription using

Method for amplifying nucleic acid sequences by strand displacement

Growing community of inventors

Lyons, France

Philippe L Cleuziat