Agence Nationale De Valorisation De La Recherche (anvar)

Philippe Fournier

Henri Heslot

Michel M Guerineau

Claude Gerbaud

Hughes Blanc

Michel Aigle

Anne Provost

Francois Lacroute

A hybrid plasmid comprising at least the DNA of a bacterial plasmid, all or part of the DNA of the 2.mu. plasmid of yeast and a segment of DNA including the gene URA.sub.3.sup.+ of yeast. These plasmids are useful as vectors of exogenous DNA in yeasts.

Hybrid plasmids and microorganisms containing them

The cell walls are removed from two distinct auxotropic mutant strains of yeast. The protoplasts thus obtained are fused and the cell walls regenerated. The strains obtained are cultured on a medium where the original auxotrophs cannot develop and the hybrid or recombinant strains are recovered. The strains obtained by the process are useful for preparing yeast proteins.

Process for obtaining hybrid strains of yeasts

Growing community of inventors

Versailles, France

Philippe Fournier