Pericor Science, Inc.

Other

Centre National De La Recherche Scientifique

Philippe Djian

Howard A Green

Bruce J Compton

George D Corey

Amandine Laetitia Vanessa Vanhoutteghem

The invention relates to keratinocyte culture, a keratinocyte culturing method and the use thereof for spreading living attenuated viruses. The inventive method makes it possible to obtain living attenuated viruses and vaccines containing said viruses.

Keratinocyte culturing method and the use thereof

Methods, products and kits are provided for attaching agents to a body tissue surface via microparticles using endogenous or exogenous transglutaminase. The microparticles have surface available transglutaminase substrate reactive groups. In an embodiment, the groups are part of a polymer containing at least two contiguous linked lysines or at least three contiguous linked glutamines.

Linkage of agents to body tissue using microparticles and transglutaminase

Methods, products, compositions and kits are provided for attaching agents to tissue with a linking molecule in the presence of transglutaminase. The linking molecule and/or agent is a substrate of transglutaminase. The agent can be a nonprotein or an enzyme such as cholinesterase or phosphodiesterase. The transglutaminase may be exogenously added or be endogenous in tissue. In specific embodiments, the agent is not a transglutaminase substrate and the linking molecule is a substrate for transglutaminase containing at least two contiguous linked glutamines or at least three contiguous linked lysines, and may be a polymer. A conjugate of the agent and the linking molecule may be applied to tissue, and in the presence of transglutaminase covalently bonded to the tissue via the linking molecule. A complementary linking molecule rich in lysines may be first attached to the tissue in the presence of transglutaminase, and then covalently bonded to a glutamine-containing linking molecule of the conjugate in the presence of transglutaminase. In another embodiment a linking molecule containing multiple glutamines is covalently bonded to tissue in the presence of transglutaminase, and an agent containing multiple lysines is covalently bonded to the linking molecule in the presence of transglutaminase. Alternatively, the linking molecule contains multiple lysines and the agent contains multiple glutamines. Two tissues can be sealed together by holding the tissues in contact with each other in the presence of transglutalinase.

Conjugates of agents and transglutaminase substrate linking molecules

Cosmetic compositions containing corneocyte proteins or polypeptides in a cosmetically acceptable vehicle can be used to form a protective layer on skin, hair or nails. The proteins may be crosslinked, thereby forming a protective layer which mimicks the corneal layer of natural skin.

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Paris, France

Philippe Djian