Genencor International, Inc.

Genecor International, Inc.

Philippe Deweer

Antoine Amory

Antione Amory

Acid-stable and thermo-stable enzymes derived from sulfolobus species

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Pullulanase, microorganisms which produce it, processes for the

Pullulanase producing microrganisms

Expression system for novel pullulanase

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucoside bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Process for the saccharification of starch

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of micro-organisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Process for the production of novel pullulanase

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.- 1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

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Aalst, Belgium

Philippe Deweer