Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Philippe Busson

Ronnie Palmgren

Anders Larsson

Hans Berg

James Van Alstine

Nicolas Thevenin

Mats Carlsson

Mattias Algotsson

Henrik Ihre

Johan William Ohman

Tobias Söderman

Dag Lindstrom

Anders Hagvall

Eva Holmgren

Maria Brännholm

David Buckley

John Daicic

Dag Lindström

Michael Morrison

Maria Holm

Klas Allmer

The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about &#x2212;20 and 20&#xb0; C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.

Method for storage and stabilization of a target substance

The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.

Method of preparing a separation matrix

The invention is a method of producing a cross-linked polymeric support having a multimodal pore structure, which comprises providing a degradable initiator molecule; providing an organic phase comprising said initiator molecule, radically polymerizable monomers and a porogen in a solvent; providing an aqueous phase comprising a transition metal catalyst; suspension polymerization of the organic phase by adding a ligand, co-ordinating to the transition metal in the aqueous phase to produce a cross-linked polymeric support having a primary pore structure and comprising initiator molecule; and subjecting the support obtained to degrading conditions to remove the initiator molecule from within the support to produce a cross-linked polymeric support having a secondary pore structure in addition to the primary pore structure.

Polymeric support having novel pore structures

The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps:

Method for synthesis of acrylamide derivatives

The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washing said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic binding groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.

Chromatographic two-layer particles

The present invention relates to a method of producing one or more macroporous cross-linked polymer particles, which comprises polymerization and cross-linking of divinyl ether monomers in an inert solvent, in which method the polymerization is free radical initiated. The invention also relates to particles so produced, which are useful as separation medium e.g. in RPC or, after suitable derivatization, in other chromatographic methods.

Method of producing macroporous cross-linked polymer particles

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Uppsala, Sweden

Philippe Busson