Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.

Philipp Ciba

Charli Kruse

Guenter R Fuhr

Daniel Hans Rapoport

Bianka Grunow

Günter Fuhr

Daniel Rapoport

The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comprises the following steps: a) mechanical comminution and/or partial enzymatic digestion of fish embryos or fish larvae; b) transfer of the comminuted and/or partially digested fish tissue into an enzyme-free medium and removing the supernatant by centrifuging to obtain a cell pellet; c) re-suspension of the cell pellet in a cell culture medium; d) culturing the cells, with the medium being changed at least once, until spontaneously contracting cell aggregates form.

Spontaneously contracting fish cell aggregates, use thereof and method for the production thereof

A method for forming a three-dimensional cell arrangement () of biological cells is disclosed, including the steps of preparation of the cell arrangement () on a flexible substrate () and deformation of the substrate (), wherein the deformation of the substrate () is brought about by an attractive force exerted by the cells () on the substrate (). A substrate (), is also disclosed, made from a flexible material and including a substrate surface () for adhesion of a cell arrangement () of biological cells, wherein the substrate surface () has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate () and the substrate () has a flexibility such that the substrate () is deformable with the action of the attraction force.

Method and device for forming a three-dimensional arrangement of biological cells

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Luebeck, Germany

Philipp Ciba