Amersham International Plc

Amersham Pharmacia Biotech UK Limited

Lgc Genomics Limited

Kbiosciences Limited

Philip Steven Robinson

Michael Alan Reeve

John Edmond Holme

Nisha Jain

Stuart Ball

John Holme

The present invention relates to methods and kits for nucleic acid detection in an assay system.

Polymerase chain reaction detection system

The present invention provides a detection system for a PCR process using FRET which comprises at least two single-labelled Oligonucleotide sequences of differing Tm that hybridize to one another in free solution to form a fluorescent quenched pair, that upon introduction of a complementary sequence to one or both sequences generates a measurable signal, one of the sequences being of a Tm that is below the Ta of the PCR process, the other not being below the Ta of the PCR process.

Detection system for PCR assay

A method for primer walking cycle sequencing of nucleic acid is provided using a presynthesized set of walking primers wherein the primers have a raised annealing temperature and/or improved annealing properties.

Primer walking cycle sequencing

Primer walking cycle sequencing using presynthesized primers containing

Fluorescently labelled dideoxynucleoside triphosphates are widely used as chain-terminators in Sanger dideoxy sequencing operations. But these unincorporated dye-terminators migrate in an electrophoresis gel and obscure the desired sequence ladder. This invention provides a method and a kit for modifying the unincorporated dye-terminators, e.g. by removal of a 5'-triphosphate group by chemical or enzymatic means e.g. by use of a phosphatase enzyme.

Modifying nucleotide analogues

Magnetic bead precipitation method

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Aylesbury, United Kingdom

Philip Steven Robinson