Standard Brands Incorporated

Philip Rotheim

Robert O Horwath

John Anthony Lally

R Otto Horwath

Thermophilic microorganisms which produce .alpha.-1,6 glucosidases are grown under aerobic conditions in an aqueous nutrient medium containing suitable carbohydrate sources. The medium is maintained at a suitable pH and, typically, at a temperature above about 40.degree. C to provide substantial growth of the microorganisms and production of .alpha.-1,6 glucosidases.

Process for producing .alpha.-1,6 glucosidases using thermophilic

Microorganisms which produce isoamylase are propagated under aerobic conditions in an aqueous nutrient medium having present maltitol. The presence of the maltitol induces the microorganisms to produce greater amounts of isoamylase as compared to the amounts of isoamylase produced by propagating the microorganisms without the presence of maltitol.

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Stamford, CT, United States of America

Philip Rotheim