Cornell Research Foundation Inc.

Sloan-kettering Institute for Cancer Research

Other

Rutgers, The State University of New Jersey

Philip Paty

Francis Barany

Monib Zirvi

Norman P Gerry

Brian W Kirk

Yu-Wei Cheng

Jianzhao Liu

Daniel Notterman

Jianzhao Kiu

The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.

Use of lecithin:retinol acyl transferase gene promoter methylation in evaluating the cancer state of subject

The present invention is directed to a method of assembling genomic maps of an organism's DNA or portions thereof. A library of an organism's DNA is provided where the individual genomic segments or sequences are found on more than one clone in the library. Representations of the genome are created, and nucleic acid sequence information is generated from the representations. The sequence information is analyzed to determine clone overlap from a representation. The clone overlap and sequence information from different representations is combined to assemble a genomic map of the organism. Once the genomic map is obtained, genomic sequence information from multiple individuals can be applied to the map and compared with one another to identify single nucleotide polymorphisms. These single nucleotide polymorphisms can be detected, and alleles quantified, by conducting (1) a global PCR amplification which creates a genome representation, and (2) a ligation detection reaction process whose ligation products are captured by hybridization to a support.

Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing

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New York, NY, United States of America

Philip Paty