Abbott Laboratories Corporation

Philip P Wang

Nai-Yi Wang

Shelley R Holets-McCormack

Thomas G Spring

Elaine Marie Brate

Marjorie A Morrison

David M Finley

David P Pacenti

Ryan E Piktel

Gloria J Hockerman

Shelley R Holets-Mccormack

Michelle Shields

The present invention is directed to a fluorescence polarization immunoassay for cyclosporin A and metabolites thereof. The present invention also relates to novel cyclosporin A derivative compounds useful in fluorescence polarization techniques. Included among the novel compounds are cyclosporin A derivatives where the amino acid in the first position is altered. The cyclosporin A derivatives are useful in forming immunogens for raising antibodies specific to cyclosporin A and metabolites thereof.

Fluorescence polarization assay for cyclosporin A and metabolites and

Fluorescence polarization assay for cyclosporin a and metabolites and

Interfering proteins are precipitated and digoxin extracted in a fluorescence polarization immunoassay for digoxin with a protein precipitating reagent. The reagent contains about 3 to 4% 5-sulfosalicyclic acid in an aqueous solution including about 40 to 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.

Precipitation of interfering proteins in fluorescence polarization

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Libertyville, IL, United States of America

Philip P Wang