Emd Millipore Corporation

University of California

Senti Biosciences, Inc.

Cell Asic

Emd Millipore Corporaticn

Philip Janmin Lee

Paul J Hung

Paul Ju-Sung Hung

Terry Gaige

Timothy Kuan-Ta Lu

Luke P Lee

Paul C Sydlowski

Russell Morrison Gordley

Brian Scott Garrison

Don-Hong Wang

Ju-Sung Paul Hung

Alba Gonzalez-Junca

Jack Tzu-Chiao Lin

Narendra Maheshri

Andrew Zayac

Wei Hsuan (Jessie) Ho

Daniel Frimannsson

Amedeo J Cappione

Amedeo Joseph Cappione, Iii

Wei Hsuan Ho

Provided herein are methods and compositions for dynamically controlling and targeting multiple immunosuppressive mechanisms in cancer. Some aspects provide cells engineered to produce multiple effector molecules, each of which modulates a different immunosuppressive mechanisms of a tumor, as well as methods of using the cells to treat cancer, such as ovarian, breast, or colon cancer.

Combinatorial cancer immunotherapy

Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.

Cell culture and invasion assay method and system

The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.

Microfluidic particle analysis method, device and system

Methods and systems are described for improved handling and/or culturing and/or assaying of cells, chemically active beads, or similar materials in microfluidic systems and microfluidic culture arrays.

Methods and apparatus for cell culture array

In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.

Connectors for pneumatic devices in microfluidic systems

A micro-incubator manifold for improved microfluidic configurations and systems and methods of manufacture and operation for a manifold and automated microfluidic systems are disclosed. Various embodiments relate to assays, systems, and/or devices for culturing cells or other biologic material in controlled environments and are applicable to related fields generally using microfluidic systems. Particular embodiments involve configurations that can be used with various standard automated handling systems, with active or passive loading and perfusion of medium and to provide high-throughput multi-assay automated systems for culturing, viewing, and analyzing cell growth, invasion, movement, chemotaxis or other properties. More specifically, specific embodiments relate to heat control systems for microfluidic culture plates and other automated systems for culture plates.

Micro-incubation systems for microfluidic cell culture and methods

A number of novel improved microfluidic configurations and systems and methods of manufacture and operation for a microfluidic invasion assay system.

Cell culture and gradient migration assay methods and devices

A valved microfluidics device, microfluidics cell-culture device and system incorporating the devices are disclosed. The valved microfluidics device includes a substrate, a microchannel through which liquid can be moved from one station to another within the device, and a pneumatic microvalve adapted to be switched between open and closed states to control the flow of fluid through a microchannel. The microvalve is formed of three flexible membranes, one of which is responsive to pneumatic pressure applied to the valve and the other two of which deform to produce a more sealable channel cross-section. The cell culture device provides valving to allow controlled loading of cells into the individual well of the device, and exchange of cell-culture components in the wells.

Valved, microwell cell-culture device and method

A number of novel improved microfluidic configurations and systems and methods of manufacture and operation. In one embodiment, three wells are used for independent cell culture systems in a cell culture array. In a second aspect, artificial sinusoids with artificial epithelial barriers are provided with just one (optionally shared or multiplexed) fluidic inlet and one (optionally shared or multiplexed) fluidic output, where the medium output also functions as a cellular input. A pneumatic cell loader combined with other components provides a fully automated cell culture system. Magnetic alignment of plate molds provides advantages and ease of molded manufacture.

Cell culture array system for automated assays and methods of operation and manufacture

The device and method relate to microfluidic particle analysis. The device is designed for trapping particles for solution analysis. Exemplary particles include beads and cells. The device has a microfluidics body, and a microfluidics channel formed in the body for receiving particles at an upstream region thereof. The channel has a deformable wall portion that defines a particle-capture region, and is responsive to a change in fluid pressure applied thereto, to selectively vary the particle-flow dimensions of the capture region. In this way, particles having a given size may be selectively retained in the capture region. In one embodiment, the deformable wall portion is expandable in response to a positive fluid pressure applied within the channel. In another embodiment, the deformable wall portion is expandable in response to a negative pressure applied to a cavity communicating with the wall portion, external to the channel.

Growing community of inventors

Alameda, CA, United States of America

Philip Janmin Lee