Commonwealth Scientific and Industrial Research Organisation

Gene Shears Pty. Limited

Philip Hendry

Philip Anthony Jennings

Maxine June McCall

Trevor John Lockett

Maxine June Mc Call

David Midgley

David Lambourne

Jason Francis Conaty

Masine J McCall

Process for stimulating and maintaining the activity of a microbial consortia within a subterranean solid carbonaceous medium to produce methane are described. The process comprises the steps of: (A) dosing a first nutrient composition into the microbial consortia environment, (B) monitoring the microbial consortia environment, including the generation of methane therein; (C) dosing a second nutrient composition into the microbial consortia based upon the results of step (B); and (D) repeating steps (B) and (C) and, if required, (E) dosing a further nutrient composition into the microbial consortia environment based upon the results of step (D).

Process of sustaining methane production in a subterranean carbonaceous medium

A nutrient composition for enhancing biogenic methane production from a carbonaceous material is described. The nutrient composition comprises a source of phosphorus (P) and a source of nitrogen (N), wherein the molar ratio of phosphorus to nitrogen (P/N) is greater than 1.5, and the nitrogen concentration is at least 0.1 m M and less than 1.7 m M. A process for enhancing biogenic methane production from a carbonaceous material is also described. The process involves contacting the nutrient composition of the invention with the carbonaceous material for a period of time to biogenically produce methane and subsequently collecting methane from the carbonaceous material. The process may further comprise contacting the carbonaceous material with a second nutrient composition, wherein the second nutrient composition has a P/N molar ratio greater than the P/N molar ratio of the former nutrient composition.

Process for enhancing biogenic methane production from a carbonaceous material using a nutrient composition with low nitrogen/high phosphorus concentrations

A nutrient combination for enhancing biogenic methane production from a carbonaceous material is described. The nutrient combination comprises a source of phosphorus (P) and gaseous nitrogen (N). The nutrient combination is preferably substantially fee of gaseous oxygen and/or gaseous NOand/or SO. In various embodiments the nutrient combination may comprise a two-phase mixture of a solution of the soluble source of phosphorus (P) and gaseous nitrogen (N). A process for enhancing biogenic methane production from a carbonaceous material is also described. The process involves dispersing the nutrient combination of the invention throughout the carbonaceous material for a period of time to biogenically produce methane and subsequently collecting methane from the carbonaceous material.

Process for enhancing biogenic methane production from a carbonaceous material using gaseous nitrogen combinations

This invention is directed to a class of miniribozymes, capable of hybridizing with a target RNA to be cleaved and exhibiting very high cleavage rates at low Mg concentration. The miniribozymes may be used in vitro or in vivo. They may be used as diagnostic or therapeutic agents.

Miniribozymes active at low magnesium ion concentrations

The invention describes catalytic nucleic acid based compounds capable of cleaving nucleic acid polymers both in vivo and in vitro. Two embodiments of this invention are compounds with a short stem that does not base pair, a minizyme, and compounds with DNA hybridizing arms and RNA catalytic domain and stem, DNA-armed ribozymes. The compounds of this invention, while nucleotide based may be substituted or modified in the sugar, phosphate, or base. Methods of use and methods of treatment are also described.

DNA-Armed ribozymes and minizymes

This invention is directed to improved catalytic compounds, minizymes and miniribozymes, capable of hybridizing with a target RNA to be cleaved. The minizymes and miniribozymes and compositions of the present invention may be used in vitro or in vivo. They may be used as diagnostic or therapeutic agents.

Optimized minizymes and miniribozymes and uses thereof

This invention is directed to a compound having the formula:

Asymmetric hammerhead ribozymes

DNA-armed ribozymes and minizymes

This invention provides catalytic molecules capable of cleaving target nucleotide sequences. More specifically, the invention provides an endonuclease having nucleotide sequences which are of sufficient length to allow hybridisation to a target nucleotide sequence desired to be cleaved. The endonuclease contains a catalytic region comprising ribonucleotides and/or deoxyribonucleotides, or derivatives thereof which act to cleave a phosphodiester bond of the substrate nucleotide sequence. The catalytic region comprises nucleotides or derivatives thereof which are linked by linking groups which may comprise ribonucleotides, deoxyribonucleotides or combinations thereof. The endonucleases of the invention are useful in the cleavage of target RNAs associated with disease in humans and animals and in the inactivation of RNA transcripts in eukaryotic and prokaryotic cells, as well as the cleavage of RNA transcripts in-vitro.

Nucleotide based endonucleases

This invention provides catalytic molecules capable of cleaving target nucleotide sequences. More specifically, the invention provides an endonuclease having nucleotide sequences which are of sufficient length to allow hybridization to a target nucleotide sequence desired to be cleaved. The endonuclease contains a catalytic region comprising ribonucleotides and/or deoxyribonucleotides, or derivatives thereof which act to cleave a phosphodiester bond of the substrate nucleotide sequence. The catalytic region comprises nucleotides or derivatives thereof which are linked by linking groups which may comprise ribonucleotides, deoxyribonucleotides or combinations thereof. The endonucleases of the invention are useful in the cleavage of target RNAs associated with disease in humans and animals and in the inactivation of RNA transcripts in eukaryotic and prokaryotic cells, as well as the cleavage of RNA transcripts in-vitro.

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Leichhardt, Australia

Philip Hendry