Thermogenesis Corporation

New York Blood Center, Inc.

Instacool Inc of North America

Syngen, Inc.

Depuy Synthes Products, Inc.

Microaire Surgical Instruments, Inc.

Baxter International Inc.

Cesca Therapeutics, Inc.

Philip H Coelho

Terry L Wolf

Pablo Rubinstein

Eric Sommer

Phil Kingsley

John Raymond Chapman

Cladd E Stevens

William Busa

Jonathan Ellis

Bruce A Baker

Richard Klosinski

Gail Rock

James H Godsey

Jim Brausch

Jim Hobbs

Rodney Sparks

Michael Lehmicke

Junzhi Li

Brian G Malone

Prince Emmanuel

Robert S Childers

Dalip Sethi

Jerry M Alcone

Richard Franklin Huyser

Ludwig Wolf, Jr

William R Bratten

Allen R Wons

Jeffery D Arnett

David P Fisher

Kevin Pelletier

John Gard

Mark Clifford Buckley

Pete J Menke

Loren Stirling

Pete Menke

Sona B Frausto

Trista K Madsen

Curtis D Mau

Peter Menke

Pablo Rubenstein

The present disclosure is directed to a method of preparing an osteogenic bone graft. The method includes introducing an anticoagulant compound into a volume of cellular material including a mononuclear cell population from the intramedullary canal of a long bone; collecting an effluent including the volume of cellular material containing the anticoagulant compound at a collection point; separating a concentrated cell fraction from the effluent, the concentrated cell fraction including the mononuclear cell population and the anticoagulant compound; and preparing an osteogenic bone graft from the concentrated cell fraction.

Method of preparing an osteogenic bone graft

Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixture to generate a stratified diluted buoyant reagent-attached target cell mixture; removing the buoyant reagent-attached target cells from the stratified diluted buoyant reagent-attached target cell mixture; and isolating the target cells from the buoyant reagent-attached target cells.

Methods for isolating target cells from blood

Disclosed herein are methods for contacting in a closed container a host liquid including target cells, microbubble reagents comprising gas-core lipid-shelled microbubbles, and one or more antibodies or other ligands that bind to cell surface molecules on the target cells, wherein the one or more antibodies or other ligands are bound to the target cells or the microbubbles, wherein the contacting under conditions to produce target cells linked to microbubbles via the one or more antibodies or other ligands and activating the target cells to generate activated target cells.

Buoyancy-activated cell sorting (BACS)-compatible activation/transduction systems and methods

An apparatus and related method for the optimization of the cryopreservation of various types of cells, including but not limited to hematopoietic and mesenchymal stem and progenitor cells, and endothelial progenitor cells found in normal blood, placental/cord blood, bone marrow or the stromal vascular fraction of adipose tissue. The apparatus for cryopreservation of biological materials in a sterile environment comprises a cryopreservation workstation, a rigid disposable cartridge, and a freezing bag assembly. The apparatus and related method provide for the precise, temperature controlled, homogenously distributed introduction of a cryoprotectant containing dimethyl sulfoxide (DMSO) into a solution containing nucleated cells in a manner that provides a safe increase in osmotic pressure and a controlled release of exothermic heat within the cells as the DMSO penetrates the cell membrane and replaces water molecules prior to the freezing of the cells.

Method and apparatus for cryopreservation of blood cells in a sterile environment

Disclosed herein are cell separation devices, methods and systems, as well as compositions and reagents for use in cell separation methods.

Cell separation devices, systems, and methods

A method for purifying and harvesting certain cell populations in blood or bone marrow by depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue is disclosed. The apparatus comprises a sterile, single use rigid, self-supporting cartridge within which the automated depletion, purification and harvesting of target cell populations occurs and all components may be distributed.

Method for purifying certain cell populations in blood or bone marrow by depleting others

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Centrifuge and separation vessel therefore

Method for separating a sample into density specific fractions

The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible.

Stem and progenitor cell compositions recovered from bone marrow or cord blood; system and method for preparation thereof

An apparatus and method for purifying and harvesting certain cell populations in blood or bone marrow by depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue is disclosed. The apparatus comprises a sterile, single use rigid, self-supporting cartridge within which the automated depletion, purification and harvesting of target cell populations occurs and all components may be distributed.

System for purifying certain cell populations in blood or bone marrow by depleting others

An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into a device adapted to fit into a centrifuge for separation of components. Each component is then sequentially extracted according to density, with a sensor present in the device to control the operation of valves directing the collection of each component. The sensor may be reading one or more of the following characteristics: infrared, optics, density, weight, radioactive, fluorescence, color, magnetism, ultrasonic, capacitance wherein the characteristic is inherent in the blood and blood component or is an additive. Each component may then be separated into its own storage container. The preferred sensors include optics and weight. Besides blood density separation, the device may contain a solution including cells, proteins, subcellular particles or viruses which may be mixed with affinity media or antibodies prior to separation.

Instrumentality for sequestering liquids based on density:method and apparatus

An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into container adapted to fit into a centrifuge for separation of components. Each component is then sequentially extracted according to density, with a sensor present in the container to control the operation of valves directing the collection of each component. Each component may then be separated into its own storage container.

Blood component separation method and apparatus

Growing community of inventors

Sacramento, CA, United States of America

Philip H Coelho