Absci LLC

Philip Barish

Sean McClain

Mark Valasek

Jeremy Minshull

The present invention provides host cells having an oxidizing cytoplasm, which are capable of growing to high cell densities and producing recombinant proteins in soluble form. Also provided are methods for growing such host cells to high densities, methods for optionally modulating the growth rates of the host cells, and methods for inducing the host cells to produce the desired gene product in soluble form. Further aspects of the invention relate to host cell preparation and storage methods, which utilize the advantages of producing gene products in host cell cytoplasm to provide methods for long-term storage and/or transport of expressed gene products that are retained in a stable and soluble form within the host cells.

Cytoplasmic expression system

The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.

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Portland, OR, United States of America

Philip Barish