Akonni Biosystems, Inc.

Bio-rad Laboratories, Inc.

Phil Belgrader

Christopher G Cooney

Bruce D Irvine

Robert Doebler

Anna Hickerson

James D Sterling

Ali Nadim

Reza Miraghaie

Kevin D Ness

Serge Saxonov

Simant Dube

Jeffrey Mellen

John Frederick Regan

Nicholas Heredia

Austin So

Mike Lucero

Ben Hindson

Bill Colston

Nicholas Jack Heredia

Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Analysis of nucleic acids

A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.

Temperature control device with a flexible temperature control surface

Methods and devices for performing chemical reactions under controlled temperatures are described. In one embodiment, the devices provided by the invention comprise a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing. The reaction chamber has thermally conductive interior and exterior surfaces defining an internal volume therein at a first temperature. The device also includes at least one thermally conductive temperature-control bladder disposed therein, which bladder is configured to receive a temperature-control substance at a second temperature into said bladder and expel said temperature-control substance from said bladder. The bladder is further configured such that upon receiving the temperature-control substance, the bladder expands to abut substantially at least a portion of said exterior surfaces of said reaction chamber to enable thermal exchange between said temperature-control substance the said internal volume of reaction chamber.

Thermal cycler for PCR including temperature control bladder

Growing community of inventors

Severna Park, MD, United States of America

Phil Belgrader