The United States of America As Represented by the Secretary of

Peter W Mason

Angray Singh Kang

Barry Baxt

Elizabeth Reider

Analia Berinstein

Marvin J Grubman

Maria Elisa Piccone

Elizabeth Rieder

A method of making a genetically-engineered cell line which is susceptible to infection by foot-and-mouth disease virus and allows the virus to replicate is disclosed. The method involves fusing the DNA encoding ICAM-1 with the DNA encoding an antibody specific for foot-and-mouth disease virus and expressing the resulting chimeric cell surface receptor protein. The chimeric cell surface receptor protein allows foot-and-mouth disease virus to bind, leading to subsequent infection and replication of foot-and-mouth disease virus. A genetically-engineered cell which expresses the chimeric cell surface receptor protein is also claimed.

Cell line for propagating receptor binding site-deleted FMDV

A foot-and-mouth disease virus has been genetically engineered by deleting the nucleic acid sequence encoding the leader (L) proteinase from an infectious cDNA copy of the viral genome and producing an L proteinase-deleted virus. The L proteinase-deleted viruses are able to assemble and grow in cells in culture, but, since they lack L proteinase, they are less toxic to infected cells within the animal, producing an attenuated infection. The recombinant virus can be formulated into an effective vaccine for the prevention of foot-and-mouth disease.

Leader-proteinase deleted foot-and-mouth disease viruses and their use

A safe, effective vaccine for the protection of susceptible animals against foot-and-mouth disease has been produced. The vaccine comprises a mutant virus from which the amino acid sequence Gly-Val-Arg-Gly-Asp-Phe (SEQ ID NO: 8) from the G-H loop of VP1 has been deleted and replaced with the amino acid sequence Asn-Pro. The mutant virus retains its antigenicity but is not infectious.

Non-infectious foot-and-mouth disease viruses

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Killingworth, CT, United States of America

Peter W Mason