Stevens Institute of Technology

Hackensack University Medical Center

New Jersey Institute of Technology

The Public Health Research Institute of the City of New York, Inc.

Peter Tolias

Woo Young Lee

Hongjun Wang

Jenny Zilberberg

Wenting Zhang

David Samuel Siegel

Timothy N Chang

Woo Lee

The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.

Ex vivo human multiple myeloma cancer niche and its use as a model for personalized treatment of multiple myeloma

Techniques for the delivery of metered amounts of liquid material include the use of a pin, such as an optical fiber. A sample of the liquid material may be placed on the tip of the pin for delivery to a target area, such as a specific location on a microarray, in a contactless manner. Light transmitted through the pin may be detected to facilitate accurate measuring of the pin's position.

Delivery of metered amounts of liquid materials

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Westfield, NJ, United States of America

Peter Tolias