Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Peter Stahl

Hans Seidel

Otto-Henning Wilhelms

Herbert Von Der Eltz

Wolfgang Gruber

Ulfert Deneke

Dolores Schendel

Winfried Albert

Herwig Brunner

Albert Roder

Wolfgang Vomel

Klaus Beaucamp

Androniki Roesch

Hans Mollering

Michael Grol

Josef Endl

Walter Schneider

Peter Wunderwald

Detlef Von Hoerschelmann

Guenther-Gerhard Jung

Edgar Meinl

Klaus Dornmair

Horst Baumgarten

The invention concerns autoreactive peptides, peptide-MHC complexes, T cell subpopulations that react thereto as well as diagnostic and therapeutic applications of these compounds.

Antigen-specific, activated T lymphocytes, detection and use

In order to detect analytes with an immunological method by incubation with receptors and analysis of the complexes formed, a mixture of at least two monoclonal antibodies which are specifically bindable to the analyte and whose cross-reactivities are different are used for at least one of the receptors.

Method for the detection of analytes

Pyrrothine derivatives and pharmaceutical use thereof

The present invention provides a process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy, based on the cellular principle, wherein leukocytes of a sample to be investigated are incubated with an allergen or another stimulation factor of degranulation, such as anti-IgE, in an aqueous medium, the leukocytes are then separated off and the remaining solution is incubated with a compound of the general formula:

Process for the detection of the presence of an allergy and for the

Process for obtaining high yields of glycerol dehydrogenase from micro-organisms and glycerol dehydrogenase having a low K.sub.M value.

Process for the recovery of glycerol dehydrogenase

The present invention provides a process for the determination of .beta.-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen. The present invention also provides a reagent for the determination of .beta.-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.

Reagent for the determination of .beta.-lactamase

In a method for the determination of glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate, with formation of .alpha.-ketoglutarate in a buffered solution, the .alpha.-ketoglutarate formed is reacted with .gamma.-aminobutyrate in the presence of .gamma.-aminobutyrate transaminase with formation of succinate semialdehyde, NADP is reduced with the latter in the presence of succinate semialdehyde dehydrogenase to give NADPH, and the latter is measured either directly or after conversion with a tetrazolium salt and an electron carrier such as diaphorase, phenantroline methosulphate or phenazine methosulphate to a formazane dye. A reagent suited for use with this method contains .gamma.-aminobutyrate transaminase, succinate semialdehyde dehydrogenase, .gamma.-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate, and optionally a tetrazolium salt, an electron carrier and a surface-active agent.

Method and reagent for the determination of glutamate-oxalacetate

A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.M

NADH Peroxidase for hydrogen peroxide determination

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Bernried, Germany

Peter Stahl