Boehringer Mannheim Gmbh

Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Herbert Von Der Eltz

Andreas Finke

Rupert Herrmann

Wolfgang Jona

Peter Sluka

Ursula Klause

Frederic Donie

Wolfgang Knoll

Hans-Georg Batz

Thomas Fischer

Alfons Nichtl

Hans Hornauer

Sebastian Heimerl

Konrad Kuerzinger

Christian Klein

Erhard Kopetzki

Oskar Nuyken

Johann Karl

Helmut Lenz

Uwe Kobold

Helmut Ringsdorf

Hans-Joachim Guder

Bernd Vogt

Albert Geiger

Hans-Ulrich Moritz

Dorothee Ambrosius

Rainer Wehner

Wolfgang Mutter

Wilhelm Kleider

Jurgen Stebani

Peter Finckh

Petra Ruger

Federic Donie

Bernd Schmidt

Dierk Beyer

Hans-Werner Griesser

Thorstein Liebermann

Manfred Zizlsperger

Hans-Dieter Kraemer

Hans-Dieter KRÄMER

Gernot Frackmann

A method of analyzing a biological sample using an analyzer and an assay. The method comprises providing the assay for producing the signal. The assay has two or more predetermined number of components. Each of the predetermined components has a distinct relation between the intensive property and the signal. The method further comprises providing calibration samples with known values for the intensive property and measuring a calibration signal for each of the calibration samples. The method further comprises determining a calibration by fitting a calibration function to the calibration signal for each of the calibration samples and the known values for the intensive property. The calibration function is equivalent to a constant plus an exponential decay term for each of the predetermined number of components. The method further comprises measuring the signal of the sample using the analyzer and the assay, and calculating the intensive property using the calibration.

Calibration for multi-component assays

The present invention relates to a method of producing a fluorescent polypeptide complex characterized in that an isolated fluorescent polypeptide is inter-molecularly cross-linked with itself or one or more other polypeptides and wherein said complex is of 40 nm to 500 nm in size, it also relates to a complex obtainable by such method, to conjugates comprising such a fluorescent polypeptide complex and to the use of such complex or conjugate.

Fluorescent polypeptide complex

Colloidal metal particles having a coating comprising non cross-linked, SH-activated dextran or aminodextran. Biomolecules may be linked directly or indirectly to the particles, which are useful as detection reagents in immunological assays.

Core-shell particles

The present invention concerns a method for producing microparticles loaded with proteins which is characterized in that the microparticles are loaded in suspension under strongly alkaline conditions. The invention also concerns microparticles which can be produced using this method and their use in a binding test e.g. in an immunoassay.

Method for producing microparticles loaded with proteins

Core-shell metal particles coated with dextran or aminodextran carrying SH groups, and use thereof

The use of polyalkylene oxide modified reagents is disclosed in a method for the detection of an analyte or in suitable reagent kits for such methods.

Polyethylene glycol-derivatized biomolecules and their use in heterogeneous detection methods

A process is described for applying spatially defined reagent areas to a solid phase which is characterized in that a liquid containing an adsorptive binding reagent is contacted with spatially defined areas of a solid phase which comprises an essentially continuous metal or metal oxide surface for an adequate time period to enable the formation of adsorptive bonds between the binding reagent and the solid phase.

Process for the application of reagent spots

A method for the detection of an analyte is described which is characterized in that the binding of the analyte to a solid phase is determined by the independent analysis of the signals from a plasmon resonance measurement and a fluorescence measurement.

Method for the simultaneous determination of biomolecular interactions by means of plasmon resonance and fluoresence detection

The invention concerns a composition comprising colloidal particles to the surface of which biomolecules are adsorbed, wherein the composition additionally contains a polyethylene glycol substituted by thiol or/and disulfide groups. This composition can be used as a detection reagent in immunological test methods.

Stabilization of metal conjugates

A surface coated with amino groups is produced by applying a polymerizable amine to a surface by means of a pulsed plasma. The coated surfaces obtained thereby have a high density of amino groups so that a specific binding phase can be obtained by covalently binding a partner of a specific binding pair to the surfaces coated with amino groups.

Process for producing a surface coated with amino groups

The invention concerns a binding matrix containing a carrier material with an oxidic surface and a solid phase reactant covalently bound thereto via anchor groups which is capable of binding to at least one free reaction partner, which is characterized in that the solid phase reactant forms a diluted and essentially laterally homogeneous binding layer on the surface of the carrier material and that the anchor groups are silane groups and are linked to the solid phase reactant via a spacer molecule.

Biotinsilane compounds and a binding matrix containing these compounds

An electrochemical sensor is provided containing a supporting material having a surface of noble metal such as gold or palladium to which is bound an enzyme such as glucose oxidase via binding molecules. The binding molecules are in the form of a homogenous monolayer and are bound to the metal surface by anchor groups such as thiol, disulphide or phosphine groups. The enzyme is linked to the binding molecules by an ionic bond, a covalent bond or a metal-chelate bond. The binding molecules may contain a spacer such as an alkylene chain. Coverage of the binding molecules on the metal surface is less than the maximum coverage to optimize enzyme coverage, decrease response time of the sensor and increase signal yield per surface area. The monolayer may contain diluent molecules which are not linked to an enzyme and which are bound to the metal surface via anchor groups. The diluent molecules lower the concentration of binding molecules in the monolayer, increase conductivity of the monolayer and lower unspecific binding of components from a sample solution in contact with the sensor. The binding molecules can be linked by an ionic bond or a metal-chelate bond, respectively, to a charged amino acid residue or a chelate-forming amino acid residue of the N-terminus, C-terminus, or N- and C-terminus of recombinant enzyme protein.

Electrochemical sensor containing an enzyme linked to binding molecules

The invention concerns the use of triazene or/and pentazadiene polymers as photoresists and in particular as positive photoresists in order to cover biochemical reagents. In addition the invention concerns a new process for the production of triazene or/and pentazadiene polymers.

Photolabile polymers with triazenedor/and pentazadiene building blocks

A method for detecting an analyte is disclosed in which the analyte is brought into contact with at least one receptor which is bound to or can be bound to a solid phase. In order to avoid unspecific interferences the addition of a glycosidic surfactant is proposed.

Method and agent for the detection of an analyte containing glycosidic

Novel compounds having the general formula

Alkyl-substituted aryl-saccharides, surface-active agents which contain

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Weilheim, Germany

Peter Sluka